Journal Article

Chemoptogenetic damage to mitochondria causes rapid telomere dysfunction

Kolodieznyi, Dmytro, Van Houten, Bennett, Roginskaya, Vera, Opresko, Patricia L., Shiva, Sruti, Bruchez, Marcel P., Kumar, Namrata, Watkins, Simon C., Qian, Wei, Fouquerel, Elise

2019

Overview

Reactive oxygen species (ROS) play important roles in aging, inflammation, and cancer. Mitochondria are an important source of ROS; however, the spatiotemporal ROS events underlying oxidative cellular damage from dysfunctional mitochondria remain unresolved. To this end, we have developed and validated a chemoptogenetic approach that uses a mitochondrially targeted fluorogen-activating peptide (Mito-FAP) to deliver a photosensitizer MG-2I dye exclusively to this organelle. Light-mediated activation (660 nm) of the Mito-FAP–MG-2I complex led to a rapid loss of mitochondrial respiration, decreased electron transport chain complex activity, and mitochondrial fragmentation. Importantly, one round of singlet oxygen produced a persistent secondary wave of mitochondrial superoxide and hydrogen peroxide lasting for over 48 h after the initial insult. By following ROS intermediates, we were able to detect hydrogen peroxide in the nucleus through ratiometric analysis of the oxidation of nuclear cysteine residues. Despite mitochondrial DNA (mtDNA) damage and nuclear oxidative stress induced by dysfunctional mitochondria, there was a lack of gross nuclear DNA strand breaks and apoptosis. Targeted telomere analysis revealed fragile telomeres and telomere loss as well as 53BP1-positive telomere dysfunction-induced foci (TIFs), indicating that DNA double-strand breaks occurred exclusively in telomeres as a direct consequence of mitochondrial dysfunction. These telomere defects activated ataxia-telangiectasia mutated (ATM)-mediated DNA damage repair signaling. Furthermore, ATM inhibition exacerbated the Mito-FAP–induced mitochondrial dysfunction and sensitized cells to apoptotic cell death. This profound sensitivity of telomeres through hydrogen peroxide induced by dysregulated mitochondria reveals a crucial mechanism of telomere–mitochondria communication underlying the pathophysiological role of mitochondrial ROS in human diseases.

• Publisher: National Academy of Sciences
• Subject: Aging / Apoptosis / Apoptosis - drug effects / Ataxia / Ataxia telangiectasia / Ataxia telangiectasia mutated protein / Biological Sciences / Cell Biology / Cell Cycle / Cell death / Cell Proliferation - drug effects / Damage / Deoxyribonucleic acid / DNA / DNA Breaks, Double-Stranded / DNA Damage / DNA Repair / DNA, Mitochondrial - metabolism / Electron transport / Electron transport chain / HEK293 Cells / Humans / Hydrogen peroxide / Hydrogen Peroxide - metabolism / Hydrogen Peroxide - toxicity / Intermediates / Membrane Potentials / Mitochondria / Mitochondria - chemistry / Mitochondria - drug effects / Mitochondria - metabolism / Mitochondrial Diseases - metabolism / Mitochondrial DNA / Oxidation / Oxidative Stress / Oxygen - metabolism / PNAS Plus / Reactive oxygen species / Reactive Oxygen Species - metabolism / Reactive Oxygen Species - toxicity / Signal Transduction / Singlet oxygen / Superoxide / Superoxides - metabolism / Superoxides - toxicity / Telomerase / Telomere - metabolism / Telomeres / Tumor Suppressor p53-Binding Protein 1 - metabolism