Journal Article

Functional Conservation in the SIAMESE-RELATED Family of Cyclin-Dependent Kinase Inhibitors in Land Plants

Bertrand, Laura L., Harashima, Hirofumi, Dassanayake, Maheshi, Beemster, Gerrit, Schnittger, Arp, Sugimoto, Keiko, Faulk, Christopher, Larkin, John C., Johnson, Matthew C., Kumar, Narender, Bramsiepe, Jonathan, Dale, Renee, Kato, Naohiro, Wang, Kai, Sizani, Bulelani L., Churchman, Michelle L., Simmons, L. Alice, Kalve, Shweta

2015

Overview

The best-characterized members of the plant-specific SIAMESE-RELATED (SMR) family of cyclin-dependent kinase inhibitors regulate the transition from the mitotic cell cycle to endoreplication, also known as endoreduplication, an altered version of the cell cycle in which DNA is replicated without cell division. Some other family members are implicated in cell cycle responses to biotic and abiotic stresses. However, the functions of most SMRs remain unknown, and the specific cyclindependent kinase complexes inhibited by SMRs are unclear. Here, we demonstrate that a diverse group of SMRs, including an SMR from the bryophyte Physcomitrella patens, can complement an Arabidopsis thaliana siamese (sim) mutant and that both Arabidopsis SIM and P. patens SMR can inhibit CDK activity in vitro. Furthermore, we show that Arabidopsis SIM can bind to and inhibit both CDKA;1 and CDKB1;1. Finally, we show that SMR2 acts to restrict cell proliferation during leaf growth in Arabidopsis and that SIM, SMR1/LGO, and SMR2 play overlapping roles in controlling the transition from cell division to endoreplication during leaf development. These results indicate that differences in SMR function in plant growth and development are primarily due to differences in transcriptional and posttranscriptional regulation, rather than to differences in fundamental biochemical function.

• Publisher: American Society of Plant Biologists,American Society of Plant Biologists (ASPB)
• Subject: Amino Acid Sequence / Arabidopsis / Arabidopsis - metabolism / Arabidopsis thaliana / Biochemistry, Molecular Biology / Biomechanical Phenomena / Cell cycle / Cell Death / Cell division / Cell growth / Cell Proliferation / Cellular Biology / Complementation / Conserved Sequence / Cyclin-Dependent Kinase Inhibitor Proteins - metabolism / Cyclins / Embryophyta - genetics / Embryophyta - metabolism / Endoreduplication / Epidermal cells / Gene Knockout Techniques / Genetic Complementation Test / Leaves / Life Sciences / Molecular biology / Molecular Sequence Data / Multigene Family / Mutation - genetics / Phenotype / Phenotypes / Phylogeny / Physcomitrella patens / Plant cells / Plant Leaves - cytology / Plant Leaves - growth & development / Plant Leaves - ultrastructure / Plant Proteins - genetics / Plant Proteins - metabolism / Protein Binding / Protoplasts - metabolism / Trichomes / Trichomes - cytology / Trichomes - metabolism / Trichomes - ultrastructure