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Community-developed checklists for publishing images and image analyses

by Chiritescu, Catalin , Schroth-Diez, Britta , Parslow, Adam C. , Montero Llopis, Paula , Nitschke, Roland , Koksoy, Ayse Aslihan , Schütz, Lucas , Seitz, Arne , Le Guyader, Sylvie , Jambor, Helena Klara , Brocher, Jan , Srinivasan, Sathya , Cimini, Beth A. , Halidi, Nadia , Hartley, Matthew , Plantard, Laure , Grunwald, David , Lacoste, Judith , Eliceiri, Kevin , Gu, Tingting , Sharma, Ved P. , Bischof, Johanna , Fernandez-Rodriguez, Julia , Miura, Kota , Boehm, Ulrike , Mathur, Aastha , Gelman, Laurent , Ecker, Rupert , Conde-Sousa, Eduardo , Ebner, Michael , Selchow, Olaf , Bertocchi, Cristina , Taatjes, Douglas , Hammer, Mathias , North, Alison , Spitaler, Martin , Held, Marie , Liu, Penghuan , Bakker, Gert-Jan , Nelson, Michael S. , Payne-Dwyer, Alex , Gaudreault, Nathalie , Tischer, Christian , Strambio-De-Castillia, Caterina , Martins, Gabriel G. , Kapoor, Varun , Le Dévédec, Sylvia , Avilov, Sergiy , Jug, Florian , Schmied, Christopher , Christopher, Jana , Ali, Rizwan , Carvalho, Mariana T. , Scott, Ryan T.

in 631/1647/328 / 706/648/479 / Annotations / Best practice / Biochemistry & Molecular Biology / Bioinformatics / Biological Microscopy / Biological Techniques / Biomedical and Life Sciences / Biomedical Engineering/Biotechnology / Biomedical research / Check lists / Checklist / Documents / Guidelines / Image processing / Image Processing, Computer-Assisted / Life Sciences / Light / Light microscopy / manipulation / Medical research / Microscopy / Molecular Biology / Molekylärbiologi / Optical microscopy / Perspective / pitfalls / platform / Proteomics / Publishing / Reproducibility / Reproducibility of Results / Software packages

2024

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Community-developed checklists for publishing images and image analyses

2024

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Community-developed checklists for publishing images and image analyses

2024

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Journal Article

Community-developed checklists for publishing images and image analyses

Chiritescu, Catalin,

Schroth-Diez, Britta,

Parslow, Adam C.,

Montero Llopis, Paula,

Nitschke, Roland,

Koksoy, Ayse Aslihan,

Schütz, Lucas,

Seitz, Arne,

Le Guyader, Sylvie,

Jambor, Helena Klara,

Brocher, Jan,

Srinivasan, Sathya,

Cimini, Beth A.,

Halidi, Nadia,

Hartley, Matthew,

Plantard, Laure,

Grunwald, David,

Lacoste, Judith,

Eliceiri, Kevin,

Gu, Tingting,

Sharma, Ved P.,

Bischof, Johanna,

Fernandez-Rodriguez, Julia,

Miura, Kota,

Boehm, Ulrike,

Mathur, Aastha,

Gelman, Laurent,

Ecker, Rupert,

Conde-Sousa, Eduardo,

Ebner, Michael,

Selchow, Olaf,

Bertocchi, Cristina,

Taatjes, Douglas,

Hammer, Mathias,

North, Alison,

Spitaler, Martin,

Held, Marie,

Liu, Penghuan,

Bakker, Gert-Jan,

Nelson, Michael S.,

Payne-Dwyer, Alex,

Gaudreault, Nathalie,

Tischer, Christian,

Strambio-De-Castillia, Caterina,

Martins, Gabriel G.,

Kapoor, Varun,

Le Dévédec, Sylvia,

Avilov, Sergiy,

Jug, Florian,

Schmied, Christopher,

Christopher, Jana,

Ali, Rizwan,

Carvalho, Mariana T.,

Scott, Ryan T.

2024

Overview

Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data. Community-developed checklists offer best-practice guidance for biologists preparing light microscopy images and describing image analyses for publications.

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Publisher

Nature Publishing Group US,Nature Publishing Group

Subject

/ Biochemistry & Molecular Biology

/ Bioinformatics

/ Biological Microscopy