Asset Details
Do you wish to reserve the book?
Structure and function of CarD, an essential mycobacterial transcription factor
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Structure and function of CarD, an essential mycobacterial transcription factor
Do you wish to request the book?
Structure and function of CarD, an essential mycobacterial transcription factor
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Structure and function of CarD, an essential mycobacterial transcription factor
2013
Overview
CarD, an essential transcription regulator in Mycobacterium tuberculosis , directly interacts with the RNA polymerase (RNAP). We used a combination of in vivo and in vitro approaches to establish that CarD is a global regulator that stimulates the formation of RNAP-holoenzyme open promoter (RPo) complexes. We determined the X-ray crystal structure of Thermus thermophilus CarD, allowing us to generate a structural model of the CarD/RPo complex. On the basis of our structural and functional analyses, we propose that CarD functions by forming protein/protein and protein/DNA interactions that bridge the RNAP to the promoter DNA. CarD appears poised to interact with a DNA structure uniquely presented by the RPo: the splayed minor groove at the double-stranded/single-stranded DNA junction at the upstream edge of the transcription bubble. Thus, CarD uses an unusual mechanism for regulating transcription, sensing the DNA conformation where transcription bubble formation initiates.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ DNA
/ Mycobacterium smegmatis - chemistry
/ Mycobacterium smegmatis - genetics
/ Mycobacterium smegmatis - metabolism
/ Mycobacterium tuberculosis - chemistry
/ Mycobacterium tuberculosis - genetics
/ Mycobacterium tuberculosis - metabolism
/ Proteins
/ RNA
/ Structure-Activity Relationship
/ Thermus
/ Transcription Factors - chemistry
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ Transcription, Genetic - physiology
