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The N-terminal hydrophobic segment of Streptomyces coelicolor FtsY forms a transmembrane structure to stabilize its membrane localization
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The N-terminal hydrophobic segment of Streptomyces coelicolor FtsY forms a transmembrane structure to stabilize its membrane localization
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Journal Article
The N-terminal hydrophobic segment of Streptomyces coelicolor FtsY forms a transmembrane structure to stabilize its membrane localization
2012
Overview
Abstract
FtsY is the receptor of the signal recognition particle that mediates the targeting of integral membrane proteins in bacteria. It was shown that in Escherichia coli, the N-terminal region of FtsY contributes to its interaction with the membrane, but it is not inserted into the membrane. However, this study presents evidence that in Streptomyces coelicolor, FtsY has a hydrophobic region at its N-terminus, which forms a membrane insertion structure and contributes significantly to the binding between FtsY and membrane. Through membrane protein extraction followed by immunoblotting, we demonstrated that deletion of the N-terminal residues 11–39 from the S. coelicolor FtsY (ScFtsY) drastically reduced its membrane-binding capability and that the N-terminus of ScFtsY alone was capable of targeting the soluble EGFP protein onto the membrane with high efficiency. Furthermore, in a labeling experiment with the membrane-impermeable probe Mal-PEG, the ScFtsY N-terminal region was protected by the membrane and was not labeled. This observation indicates that this region was inserted into the membrane.
Publisher
Blackwell Publishing Ltd,Wiley-Blackwell,Oxford University Press
Subject
Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Binding
/ Biological and medical sciences
/ E coli
/ FtsY
/ Fundamental and applied biological sciences. Psychology
/ Hydrophobic and Hydrophilic Interactions
/ Proteins
/ Receptors, Cytoplasmic and Nuclear - chemistry
/ Receptors, Cytoplasmic and Nuclear - genetics
/ Receptors, Cytoplasmic and Nuclear - metabolism
/ Streptomyces coelicolor - chemistry
/ Streptomyces coelicolor - genetics
