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gene from the cellulose synthase-like C family encodes a β-1,4 glucan synthase
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gene from the cellulose synthase-like C family encodes a β-1,4 glucan synthase
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Journal Article
gene from the cellulose synthase-like C family encodes a β-1,4 glucan synthase
2007
Overview
Despite the central role of xyloglucan (XyG) in plant cell wall structure and function, important details of its biosynthesis are not understood. To identify the gene(s) responsible for synthesizing the β-1,4 glucan backbone of XyG, we exploited a property of nasturtium (Tropaeolum majus) seed development. During the last stages of nasturtium seed maturation, a large amount of XyG is deposited as a reserve polysaccharide. A cDNA library was produced from mRNA isolated during the deposition of XyG, and partial sequences of 10,000 cDNA clones were determined. A single member of the C subfamily from the large family of cellulose synthase-like (CSL) genes was found to be overrepresented in the cDNA library. Heterologous expression of this gene in the yeast Pichia pastoris resulted in the production of a β-1,4 glucan, confirming that the CSLC protein has glucan synthase activity. The Arabidopsis CSLC4 gene, which is the gene with the highest sequence similarity to the nasturtium CSL gene, is coordinately expressed with other genes involved in XyG biosynthesis. These and other observations provide a compelling case that the CSLC gene family encode proteins that synthesize the XyG backbone.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Arabidopsis Proteins - chemistry
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ CSL gene
/ Enzymes
/ Gene Expression Regulation, Plant
/ genes
/ Glucans
/ Glucosyltransferases - chemistry
/ Glucosyltransferases - classification
/ Glucosyltransferases - genetics
/ Glucosyltransferases - metabolism
/ Plants
/ Reverse Transcriptase Polymerase Chain Reaction
/ seeds
/ Seeds - growth & development
/ Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/ Yeasts
