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Journal Article
高脂饮食结合球囊损伤快速建立ApoE-/-大鼠动脉粥样硬化模型
2016
Overview
目的探索利用短期高脂饮食结合球囊损伤的造模方式，快速建立载脂蛋白E基因敲除（ApoE-/-小）大鼠动脉粥样硬化（As）动物模型。方法选择8周雄性SD大鼠（n=10）及ApoE-/-大鼠（n=10），高脂饮食2周后分别检测两组血常规、肝肾功能、血脂谱及C反应蛋白（CRP）等指标。随后行大鼠左侧颈总动脉球囊损伤术，2周后采用过量水合氯醛处死大鼠，取术侧颈总动脉进行HE、Masson及油红O染色，并行CD68、α平滑肌蛋白（α-SMA）免疫荧光染色。结果ApoE-/-大鼠血脂水平明显高于sD大鼠[总胆固醇（TC）：18．56±2．82mmol／Lvs5．69±1.98mmol／L，P〈0．01；低密度脂蛋白（LDL）：6．86±1．47mmol／Lvs 1．92±0．76mmol／L，P〈0．01]；与SD大鼠比较，在严重血脂紊乱状态下，ApoE-/-手大鼠全身处于显著炎症状态[CRP：4．66±0．57mg／L1）so．39±0．21mg／L，P〈0．05；白细胞：（21．79±5．10）×10^9／Lvs（14．82±2．41）×10^9／L，P〈0．01；中性粒细胞：（9．28±3．35）×10^9／Lvs（2．10±0．96）×10^9／L，P〈0．01]。HE及Masson染色结果显示，两组均有显著增生形成，并伴随着胶原纤维的轻度沉积；油红O染色可见ApoE-/-大鼠斑块增生明显，而SD大鼠呈阴性。免疫荧光染色结果显示，ApoE-/-大鼠斑块内CD68呈显著阳性，α-SMA呈弱阳性，而sD大鼠α-SMA呈阳性，无明显CD68显色。结论通过短期高脂饮食结合球囊损伤的造模方式，可以快速建立ApoE-/-小大鼠AS模型。
