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Single‐Cell Encapsulation via Click‐Chemistry Alters Production of Paracrine Factors from Neural Progenitor Cells
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Single‐Cell Encapsulation via Click‐Chemistry Alters Production of Paracrine Factors from Neural Progenitor Cells
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Single‐Cell Encapsulation via Click‐Chemistry Alters Production of Paracrine Factors from Neural Progenitor Cells
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Journal Article
Single‐Cell Encapsulation via Click‐Chemistry Alters Production of Paracrine Factors from Neural Progenitor Cells
2020
Overview
Extracellular matrix (ECM) properties affect multiple cellular processes such as cell survival, proliferation, and protein synthesis. Thus, a polymeric‐cell delivery system with the ability to manipulate the extracellular environment can act as a fundamental regulator of cell function. Given the promise of stem cell therapeutics, a method to uniformly enhance stem cell function, in particular trophic factor release, can prove transformative in improving efficacy and increasing feasibility by reducing the total number of cells required. Herein, a click‐chemistry powered 3D, single‐cell encapsulation method aimed at synthesizing a polymeric coating with the optimal thickness around neural progenitor cells is introduced. Polymer encapsulation of neural stem cells significantly increases the release of neurotrophic factors such as VEGF and CNTF. Cell encapsulation with a soft extracellular polymer upregulates the ADCY8‐cAMP pathway, suggesting a mechanism for the increase in paracrine factors. Hence, the described single‐cell encapsulation technique can emerge as a translatable, nonviral cell modulation method and has the potential to improve stem cells' therapeutic effect. Through the use of cell glycoengineering techniques and click chemistry, single‐cell polymer encapsulation is possible. This polymeric extracellular matrix can modify the trophic factor production of neural progenitor cells. The cyclic adenosine monophosphate pathway and actin interactions are important in trophic factor regulation, specifically for VEGFB. The ability to encapsulate cells has implications for optimizing stem cell therapeutics.
