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Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum
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Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum
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Journal Article
Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum
2016
Overview
Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.
Publisher
American Association for the Advancement of Science,The American Association for the Advancement of Science,American Association for the Advancement of Science (AAAS)
Subject
/ Dynamics
/ Enzymes
/ Glucosyltransferases - chemistry
/ Glucosyltransferases - isolation & purification
/ Glucosyltransferases - metabolism
/ Highways
/ Lipids - isolation & purification
/ Luminescent Proteins - analysis
/ Luminescent Proteins - chemistry
/ Multienzyme Complexes - chemistry
/ Multienzyme Complexes - isolation & purification
/ Multienzyme Complexes - metabolism
/ Plant Proteins - isolation & purification
/ Sorghum
