Journal Article

Biosynthetic Pathway for Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

Liu, Changshui, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P. R. China, Wang, Qi, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P. R. China, Zhang, Yongguang, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P. R. China, Zhao, Guang, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P. R. China, Xian, Mo, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P. R. China

2012

Overview

Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, we engineered a P3HP biosynthetic pathway in recombinant Escherichia coli. The genes for malonyl-CoA reductase (mcr, from Chloroflexus aurantiacus), propionyl-CoA synthetase (prpE, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Ralstonia eutropha) were cloned and expressed in E. coli. The E. coli genes accABCD encoding acetyl-CoA carboxylase were used to channel the carbon into the P3HP pathway. Using glucose as a sole carbon source, the cell yield and P3HP content were 1.32 g/L and 0.98% (wt/wt [cell dry weight]), respectively. Although the yield is relatively low, our study shows the feasibility of engineering a P3HP biosynthetic pathway using a structurally unrelated carbon source in bacteria.

• Publisher: The Microbiological Society of Korea,Springer Nature B.V,한국미생물학회
• Subject: Acetyl-CoA carboxylase / bacteria / biochemical pathways / Biodegradability / Biodegradation / Biomedical and Life Sciences / Biopolymers - biosynthesis / Biosynthesis / Biosynthetic Pathways - genetics / Carbon / Carbon - metabolism / Carbon sources / Chloroflexus - enzymology / Chloroflexus - genetics / Chloroflexus aurantiacus / Cloning / Culture Media - chemistry / Cupriavidus necator / Cupriavidus necator - enzymology / Cupriavidus necator - genetics / E coli / engineering / Enzymes / ESCHERICHIA COLI / Escherichia coli - genetics / Escherichia coli - metabolism / Genes / Glucose / Glucose - metabolism / Glycerol / Lactic Acid - analogs & derivatives / Lactic Acid - biosynthesis / Life Sciences / malonyl coenzyme A / malonyl-CoA reductase / Metabolic Engineering / Metabolism / Microbiology / Plasmids / polyhydroxyalkanoate synthase / Polyhydroxyalkanoates / Polyhydroxyalkanoic acid / propionyl-coenzyme A synthetase / Ralstonia eutropha / recombinant E. coli / reductase / thermoplastics / 생물학