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Expression of the genes for lysostaphin and lysostaphin resistance in streptococci
by
Heath Farris, M
, Heath, Lucie S
, Sloan, Gary L
, Heath, Harry E
, LeBlanc, Paul A
, Simmonds, Robin S
in
Bacterial Proteins - metabolism
/ Bacteriology
/ Biological and medical sciences
/ Cloning, Molecular
/ Endopeptidases - genetics
/ Endopeptidases - metabolism
/ epr gene
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation, Bacterial
/ Gene Expression Regulation, Enzymologic
/ Genetics
/ Lysostaphin
/ Lysostaphin - metabolism
/ lysostaphin endopeptidase
/ Microbiology
/ Miscellaneous
/ Peptidoglycan hydrolase
/ R Factors
/ Staphylococcus simulans
/ Staphylococcus simulans biovar staphylolyticus
/ Streptococcus
/ Streptococcus equi
/ Streptococcus equi - enzymology
/ Streptococcus equi - genetics
/ Streptococcus equi subsp. zooepidemicus
/ Virology
/ Zoocin A
2003
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Expression of the genes for lysostaphin and lysostaphin resistance in streptococci
by
Heath Farris, M
, Heath, Lucie S
, Sloan, Gary L
, Heath, Harry E
, LeBlanc, Paul A
, Simmonds, Robin S
in
Bacterial Proteins - metabolism
/ Bacteriology
/ Biological and medical sciences
/ Cloning, Molecular
/ Endopeptidases - genetics
/ Endopeptidases - metabolism
/ epr gene
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation, Bacterial
/ Gene Expression Regulation, Enzymologic
/ Genetics
/ Lysostaphin
/ Lysostaphin - metabolism
/ lysostaphin endopeptidase
/ Microbiology
/ Miscellaneous
/ Peptidoglycan hydrolase
/ R Factors
/ Staphylococcus simulans
/ Staphylococcus simulans biovar staphylolyticus
/ Streptococcus
/ Streptococcus equi
/ Streptococcus equi - enzymology
/ Streptococcus equi - genetics
/ Streptococcus equi subsp. zooepidemicus
/ Virology
/ Zoocin A
2003
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Expression of the genes for lysostaphin and lysostaphin resistance in streptococci
by
Heath Farris, M
, Heath, Lucie S
, Sloan, Gary L
, Heath, Harry E
, LeBlanc, Paul A
, Simmonds, Robin S
in
Bacterial Proteins - metabolism
/ Bacteriology
/ Biological and medical sciences
/ Cloning, Molecular
/ Endopeptidases - genetics
/ Endopeptidases - metabolism
/ epr gene
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation, Bacterial
/ Gene Expression Regulation, Enzymologic
/ Genetics
/ Lysostaphin
/ Lysostaphin - metabolism
/ lysostaphin endopeptidase
/ Microbiology
/ Miscellaneous
/ Peptidoglycan hydrolase
/ R Factors
/ Staphylococcus simulans
/ Staphylococcus simulans biovar staphylolyticus
/ Streptococcus
/ Streptococcus equi
/ Streptococcus equi - enzymology
/ Streptococcus equi - genetics
/ Streptococcus equi subsp. zooepidemicus
/ Virology
/ Zoocin A
2003
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Expression of the genes for lysostaphin and lysostaphin resistance in streptococci
Journal Article
Expression of the genes for lysostaphin and lysostaphin resistance in streptococci
2003
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Overview
To determine if the genes for lysostaphin endopeptidase (
end) and lysostaphin resistance (
epr) function in streptococci, we transferred these genes from
Staphylococcus simulans biovar
staphylolyticus into two strains of
Streptococcus equi subsp.
zooepidemicus. The
end-containing streptococci were able to produce and process proendopeptidase. Strains containing
epr were more resistant to lysis by the streptococcolytic enzyme zoocin A and amino acid analysis of the peptidoglycans of the
epr-containing streptococci revealed insertion of serines in their cross bridges. This is the first report of the transfer of a
femABX-like immunity factor resulting in a physiologically useful effect in a different genus.
Publisher
Elsevier B.V,Blackwell Publishing Ltd,Blackwell
Subject
Bacterial Proteins - metabolism
/ Biological and medical sciences
/ epr gene
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation, Bacterial
/ Gene Expression Regulation, Enzymologic
/ Genetics
/ Staphylococcus simulans biovar staphylolyticus
/ Streptococcus equi - enzymology
/ Streptococcus equi - genetics
/ Streptococcus equi subsp. zooepidemicus
/ Virology
/ Zoocin A
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