Asset Details
Do you wish to reserve the book?
Two Chloroflexi classes independently evolved the ability to persist on atmospheric hydrogen and carbon monoxide
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Two Chloroflexi classes independently evolved the ability to persist on atmospheric hydrogen and carbon monoxide
Do you wish to request the book?
Two Chloroflexi classes independently evolved the ability to persist on atmospheric hydrogen and carbon monoxide
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Two Chloroflexi classes independently evolved the ability to persist on atmospheric hydrogen and carbon monoxide
2019
Overview
Most aerobic bacteria exist in dormant states within natural environments. In these states, they endure adverse environmental conditions such as nutrient starvation by decreasing metabolic expenditure and using alternative energy sources. In this study, we investigated the energy sources that support persistence of two aerobic thermophilic strains of the environmentally widespread but understudied phylum Chloroflexi. A transcriptome study revealed that
Thermomicrobium roseum
(class Chloroflexia) extensively remodels its respiratory chain upon entry into stationary phase due to nutrient limitation. Whereas primary dehydrogenases associated with heterotrophic respiration were downregulated, putative operons encoding enzymes involved in molecular hydrogen (H
2
), carbon monoxide (CO), and sulfur compound oxidation were significantly upregulated. Gas chromatography and microsensor experiments showed that
T. roseum
aerobically respires H
2
and CO at a range of environmentally relevant concentrations to sub-atmospheric levels. Phylogenetic analysis suggests that the hydrogenases and carbon monoxide dehydrogenases mediating these processes are widely distributed in Chloroflexi genomes and have probably been horizontally acquired on more than one occasion. Consistently, we confirmed that the sporulating isolate
Thermogemmatispora
sp. T81 (class Ktedonobacteria) also oxidises atmospheric H
2
and CO during persistence, though further studies are required to determine if these findings extend to mesophilic strains. This study provides axenic culture evidence that atmospheric CO supports bacterial persistence and reports the third phylum, following Actinobacteria and Acidobacteria, to be experimentally shown to mediate the biogeochemically and ecologically important process of atmospheric H
2
oxidation. This adds to the growing body of evidence that atmospheric trace gases are dependable energy sources for bacterial persistence.
Publisher
Nature Publishing Group UK,Oxford University Press
Subject
/ 82/16
/ Aldehyde Oxidoreductases - genetics
/ Aldehyde Oxidoreductases - metabolism
/ Bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biomedical and Life Sciences
/ Carbon Monoxide - metabolism
/ Ecology
/ Electron Transport - genetics
/ Gases
/ Genome, Bacterial - genetics
/ Genomes
/ Germfree
/ Hydrogen
/ Microbial Genetics and Genomics
/ Multienzyme Complexes - genetics
/ Multienzyme Complexes - metabolism
/ Operons
/ Sulfur
