Journal Article

Mosquitoes infected with dengue viruses in Brazil

de Figueiredo, Mario LG, de C Gomes, Almério, de S Leandro, André, Figueiredo, Luiz TM, do SM Castro, Jesuína, de S Orrico, Agnaldo, Amarilla, Alberto A, Durigon, Edison L, de Araujo, Renato F, Aquino, Victor H

2010

Overview

Dengue epidemics have been reported in Brazil since 1985. The scenery has worsened in the last decade because several serotypes are circulating and producing a hyper-endemic situation, with an increase of DHF/DSS cases as well as the number of fatalities. Herein, we report dengue virus surveillance in mosquitoes using a Flavivirus genus-specific RT-Hemi-Nested-PCR assay. The mosquitoes ( Culicidae , n = 1700) collected in the Northeast, Southeast and South of Brazil, between 1999 and 2005, were grouped into 154 pools. Putative genomes of DENV-1, -2 and -3 were detected in 6 mosquito pools (3.8%). One amplicon of putative DENV-1 was detected in a pool of Haemagogus leucocelaenus suggesting that this virus could be involved in a sylvatic cycle. DENV-3 was found infecting 3 pools of larvae of Aedes albopictus and the nucleotide sequence of one of these viruses was identified as DENV-3 of genotype III, phylogenetically related to other DENV-3 isolated in Brazil. This is the first report of a nucleotide sequence of DENV-3 from larvae of Aedes albopictus .

• Publisher: BioMed Central,BMC
• Subject: Aedes albopictus / Animals / Biomedical and Life Sciences / Biomedicine / Brazil / Culicidae / Culicidae - virology / dengue / Dengue - virology / Dengue virus / Dengue Virus - classification / Dengue Virus - genetics / Dengue Virus - isolation & purification / Female / Flavivirus / genome / genotype / Haemagogus leucocelaenus / Humans / Insect Vectors - virology / Male / Molecular Sequence Data / monitoring / nucleotide sequences / Phylogeny / serotypes / Virology / viruses