Journal Article

Physiological roles of Casparian strips and suberin in the transport of water and solutes

Universität Bonn = University of Bonn, FEDER-Junta de Castilla y León, CLU-2018-04, Frenger, Marc, University of Nottingham, UK (UON), ERA-NET Coordinating Action in Plant Sciences program project ERACAPS13.089_RootBarriers, Hidalgo‐shrestha, Christopher, Dauzat, Myriam, Boursiac, Yann, European Project: 609398,EC:FP7:PEOPLE,FP7-PEOPLE-2013-COFUND,AGREENSKILLSPLUS, Universidad de Salamanca [España] = University of Salamanca [Spain], Muller, Bertrand, Calvo‐polanco, Monica, Reyt, Guilhem, ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée, Franke, Rochus, Salt, David, Ribeyre, Zoe, Diehl, Patrick, Biochimie et Physiologie Moléculaire des Plantes (BPMP) ; Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Transnational Cooperation within the PLANT-KBBE Initiative,

2021

Overview

The formation of Casparian strips (CS) and the deposition of suberin at the endodermis of plant roots are thought to limit the apoplastic transport of water and ions. We investigated the specific role of each of these apoplastic barriers in the control of hydro-mineral transport by roots and the consequences on shoot growth.A collection of Arabidopsis thaliana mutants defective in suberin deposition and/or CS development was characterized under standard conditions using a hydroponic system and the Phenopsis platform.Mutants altered in suberin deposition had enhanced root hydraulic conductivity, indicating a restrictive role for this compound in water transport. In contrast, defective CS directly increased solute leakage and indirectly reduced root hydraulic conductivity. Defective CS also led to a reduction in rosette growth, which was partly dependent on the hydro-mineral status of the plant. Ectopic suberin was shown to partially compensate for defective CS phenotypes. Altogether, our work shows that the functionality of the root apoplastic diffusion barriers greatly influences the plant physiology, and that their integrity is tightly surveyed.

• Publisher: Wiley,CCSD,Wiley Subscription Services, Inc,John Wiley and Sons Inc
• Subject: apoplastic barriers / aquaporins / Arabidopsis - genetics / Arabidopsis thaliana / Casparian strips / Cell Wall / Deposition / Diffusion barriers / endodermis / Hydroponics / Life Sciences / Lipids / Mutants / Phenotypes / physiological transport / Physiology / Plant physiology / Plant Roots / root hydraulic conductivity / Roots / Rosette / Solutes / solutes diffusion / suberin / Transport / Vegetal Biology / Water / Water transport
• ISBN: 0007094651000