Journal Article

Arabidopsis thaliana CYCLIC NUCLEOTIDE‐GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis

Davies, Julia, Wang, Limin, Institut des Sciences des Plantes de Montpellier (IPSIM) ; Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Mcnelly, Rose, Moeder, Wolfgang, Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant (PIAF) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Dark, Adeeba, UK Research and Innovation's Biotechnology and Biological Sciences Research Council (BBSRC)BB/J014540/1UK Research & Innovation (UKRI) - Biotechnology and Biological Sciences Research Council (BBSRC)BB/S004637/1National S

2022

Overview

Damage can be signalled by extracellular ATP (eATP) using plasma membrane (PM) receptors to effect cytosolic free Ca 2+ ([Ca 2+ ] cyt) increase as a second messenger. The downstream PM Ca 2+ channels remain enigmatic. Here, the Arabidopsis thaliana Ca 2+ channel subunit Cyclic Nucleotide-Gated Channel2 (CNGC2) was identified as a critical component linking eATP receptors to downstream [Ca 2+ ] cyt signalling in roots.  eATP-induced changes in single epidermal cell PM voltage and conductance were measured electrophysiologically, changes in root [Ca 2+ ] cyt were measured with aequorin and root transcriptional changes were determined by qRT-PCR. Two cngc2 loss of function mutants were used: cngc2-3 and dnd1 (which expresses cytosolic aequorin).  eATP-induced transient depolarisation of Arabidopsis root elongation zone epidermal PM voltage was Ca 2+-dependent, requiring CNGC2 but not CNGC4 (its channel co-subunit in immunity signalling). Activation of PM Ca 2+ influx currents also required CNGC2. The eATP-induced [Ca 2+ ] cyt increase and transcriptional response in cngc2 roots were significantly impaired.  CNGC2 is required for eATP-induced epidermal Ca 2+ influx, causing depolarisation leading to [Ca 2+ ] cyt increase and damage-related transcriptional response.

• Publisher: Wiley,CCSD,Wiley Subscription Services, Inc,John Wiley and Sons Inc
• Subject: Adenosine triphosphate / Adenosine Triphosphate - metabolism / aequorin / Arabidopsis / Arabidopsis - metabolism / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Arabidopsis thaliana / ATP / Calcium / Calcium - metabolism / Calcium channels / Calcium channels (voltage-gated) / Calcium influx / Calcium ions / Calcium signalling / CNGC4 / Critical components / Cyclic Nucleotide-Gated Cation Channels - genetics / Cyclic Nucleotide-Gated Cation Channels - metabolism / Cyclic Nucleotide-Gated Cation Channels - pharmacology / CYCLIC NUCLEOTIDE‐GATED CHANNEL2 (CNGC2) / Damage / Depolarization / Electric potential / electric potential difference / Elongation / Epidermal Cells / Epidermis / Epidermis - metabolism / Extracellular / extracellular ATP / Immunity / Life Sciences / loss-of-function mutation / Nucleotide sequence / Nucleotides / Nucleotides, Cyclic - metabolism / Nucleotides, Cyclic - pharmacology / PCR / plasma membrane / plasma membrane potential / quantitative polymerase chain reaction / Rapid Report / Receptors / Renilla-luciferin 2-monooxygenase / root epidermis / root growth / Roots / Signal Transduction / Signaling / Skin / Transcription / transcription (genetics) / Vegetal Biology / Voltage
• ISBN: 0007579555000