Journal Article

Cell-based skin substitutes accelerate regeneration of extensive burn wounds in rats

Hassanpour, Seyed Esmaeil, Farajzadeh-Vajari, Fatemeh, Bajouri, Amir, Sodeifi, Niloofar, Motamed, Sadrollah, Shafieyan, Saeed, Molaei, Hojjat, Azargashb, Enzollah, Taghiabadi, Ehsan, Aghdami, Nasser

2017

Overview

This study investigated the effects of amniotic membrane combined with adipose-derived stem cells or fetal fibroblasts on regenerating extensive burns in rats. Third degree burns of 1100–1800 mm2 were induced on 32 Sprague-Dawley rats. Burned sites were excised and randomly covered with Vaseline gauze (control), human amniotic membrane (HAM), human fetal fibroblasts seeded on HAM (HAM-FF), or human adipose-derived stem cells seeded on HAM (HAM-ASC), and followed by wound closure and histological assessments. Wound closure rates of HAM-FF, HAM-ASC, HAM and control groups at seven and 14 days after the treatment were 42.2% and 81.9%, 41.9% and 81.7%, 33.5% and 74.2%, and 16.5% and 69.7%, respectively. Wounds of HAM-FF, HAM-ASC, HAM and control groups were closed on 40, 40, 50 and 60 days after the treatment, respectively (P < 0.05). Histological assessments revealed lower inflammatory cell infiltration in HAM-ASC and HAM-FF groups. Cell-based engineered skin substitutes seem to accelerate wound regeneration, especially within the first 14 days.

• Publisher: Elsevier Inc,Elsevier Limited
• Subject: Adipose Tissue - cytology / Amnion / Amniotic membrane / Animals / Assessments / Burn / Burns / Burns - therapy / Cells, Cultured / Epidermal growth factor / Fetuses / Fibroblasts / Gauze / Humans / Infiltration / Inflammation / Male / Petrolatum - pharmacology / Rats / Rats, Sprague-Dawley / Regeneration / Rodents / Skin / Stem cell / Stem Cells / Surgery / Tissue Engineering / Vascular endothelial growth factor / Wound healing / Wound Healing - physiology / Wounds