Journal Article

Fullerenol C60(OH)24 Effects on Antioxidative Enzymes Activity in Irradiated Human Erythroleukemia Cell Line

Dragan ZIKIC, Aleksandar DJORDJEVIC, Ivana ICEVIC, Aleksandra NIKOLIC, Karmen STANKOV, Slavica SOLAJIC, Gordana BOGDANOVIC, Visnja BOGDANOVIC

2008

Overview

Radiotherapy-induced toxicity is a major dose-limiting factor in anti-cancer treatment. Ionizing radiation leads to the formation of reactive oxygen and nitrogen species (ROS/RNS) that are associated with radiation-induced cell death. Investigations of biological effects of fullerenol have provided evidence for its ROS/RNS scavenger properties in vitro and radioprotective efficiency in vivo. Therefore we were interested to evaluate its radioprotective properties in vitro in the human erythroleukemia cell line. Pretreatment of irradiated cells by fullerenol exerted statistically significant effects on cell numbers and the response of antioxidative enzymes to X-ray irradiation-induced oxidative stress in cells. Our study provides evidence that the pre-treatment with fullerenol enhanced the enzymatic activity of superoxide dismutase and glutathione peroxidase in irradiated K562 cells.

• Publisher: THE JAPAN RADIATION RESEARCH SOCIETY,The Japan Radiation Research Society and Japanese Society for Therapeutic Radiology and Oncology,Oxford University Press
• Subject: Biocompatibility / Biological effects / Cell death / Enzymes / Free Radical Scavengers - pharmacology / Fullerenes - pharmacology / Glutathione / Glutathione Peroxidase - metabolism / Humans / Ionizing radiation / K562 Cells / Leukemia, Erythroblastic, Acute - enzymology / Peroxidase / Pretreatment / Radiation effects / Radiation therapy / Radiation-Protective Agents - pharmacology / Superoxide dismutase / Superoxide Dismutase - metabolism / X ray irradiation