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Noc protein binds to specific DNA sequences to coordinate cell division with chromosome segregation
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Journal Article
Noc protein binds to specific DNA sequences to coordinate cell division with chromosome segregation
2009
Overview
Coordination of chromosome segregation and cytokinesis is crucial for efficient cell proliferation. In
Bacillus subtilis
, the nucleoid occlusion protein Noc protects the chromosomes by associating with the chromosome and preventing cell division in its vicinity. Using protein localization, ChAP‐on‐Chip and bioinformatics, we have identified a consensus Noc‐binding DNA sequence (NBS), and have shown that Noc is targeted to about 70 discrete regions scattered around the chromosome, though absent from a large region around the replication terminus. Purified Noc bound specifically to an NBS
in vitro
. NBSs inserted near the replication terminus bound Noc–YFP and caused a delay in cell division. An autonomous plasmid carrying an NBS array recruited Noc–YFP and conferred a severe Noc‐dependent inhibition of cell division. This shows that Noc is a potent inhibitor of division, but that its activity is strictly localized by the interaction with NBS sites
in vivo
. We propose that Noc serves not only as a spatial regulator of cell division to protect the nucleoid, but also as a timing device with an important role in the coordination of chromosome segregation and cell division.
Publisher
John Wiley & Sons, Ltd,Nature Publishing Group UK,Springer Nature B.V,Nature Publishing Group
Subject
/ Bacillus subtilis - physiology
/ Bacterial Proteins - analysis
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ chromosome replication/segregation
/ Chromosomes, Bacterial - genetics
/ Chromosomes, Bacterial - metabolism
/ coordination with cell division
/ DNA
/ DNA-Binding Proteins - analysis
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ EMBO06
/ EMBO23
/ Genomics
/ Noc
/ Proteins
