Journal Article

Targeting cancer stem cells in medulloblastoma by inhibiting AMBRA1 dual function in autophagy and STAT3 signalling

Locatelli, Franco, Ferretti Elisabetta, Gianesello Matteo, De Luca Carmen Dolores, Caruso, Simona, Ceglie Donatella, Abballe Luana, Caruana Ignazio, Ballabio Claudio, Vinci, Maria, Milletti Giacomo, Camera, Antonio, Velardi Enrico, Nazio Francesca, Marinelli, Sara, Cecconi, Francesco, Pezzullo, Marco, Po Agnese, Miele Evelina, Bordi Matteo, Quintarelli Concetta, Campello, Silvia, Cacchione Antonella, Tiberi Luca, Papaleo, Elena, Reddel Sofia, Mastronuzzi, Angela, Ferraina Caterina, Carai, Andrea, De Angelis Biagio, Diomedi, Camassei Francesca

2021

Overview

Medulloblastoma (MB) is a childhood malignant brain tumour comprising four main subgroups characterized by different genetic alterations and rate of mortality. Among MB subgroups, patients with enhanced levels of the c-MYC oncogene (MBGroup3) have the poorest prognosis. Here we identify a previously unrecognized role of the pro-autophagy factor AMBRA1 in regulating MB. We demonstrate that AMBRA1 expression depends on c-MYC levels and correlates with Group 3 patient poor prognosis; also, knockdown of AMBRA1 reduces MB stem potential, growth and migration of MBGroup3 stem cells. At a molecular level, AMBRA1 mediates these effects by suppressing SOCS3, an inhibitor of STAT3 activation. Importantly, pharmacological inhibition of autophagy profoundly affects both stem and invasion potential of MBGroup3 stem cells, and a combined anti-autophagy and anti-STAT3 approach impacts the MBGroup3 outcome. Taken together, our data support the c-MYC/AMBRA1/STAT3 axis as a strong oncogenic signalling pathway with significance for both patient stratification strategies and targeted treatments of MBGroup3.

• Publisher: Springer Nature B.V
• Subject: Autophagy / Brain cancer / Brain tumors / c-Myc protein / Cell migration / Children / Medulloblastoma / Myc protein / Phagocytosis / Prognosis / Signal transduction / Stat3 protein / Stem cells