Journal Article

Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species

Choi, Hee-Seung, Kang, Byoung-Cheorl, Hill, Theresa, Kim, Yeon-Ki, Sørensen, Iben, Park, Minkyu, Kwon, Suk-Yoon, Oh, Sang-Keun, Yu, Yeisoo, Lee, Yong-Hwan, Cheong, Kyeongchae, Lee, Min-Soo, Lim, Jong-Sung, Yang, Hee-Bum, Choi, Beom-Soon, Bae, Chungyun, Kim, Byung-Dong, Suh, Mi Chung, Kim, Shin-Young, Kim, Myung-Shin, Choi, Ik-Young, Noh, Seung-Jae, Bosland, Paul W, Kim, Woo Taek, Choi, Sang-Bong, Cho, Hyung-Taeg, Kim, Ryan W, Yeam, Inhwa, Kim, Hyun-Jin, Ahn, Hee Kyung, Lee, Hyun-Ah, Kwon, Jin-Kyung, Kim, Hyun A, Lee, Sang-Jik, Jo, Sung-Hwan, Kim, Saet-Byul, Lee, Saet Buyl, Lee, Gir-Won, Jeong, Hee-Jin, Park, June Hyun, Pai, Hyun-Sook, Giovannoni, James J, Shin, Younhee, Rose, Jocelyn K C, Do Choi, Yang, Yeom, Seon-In, Lee, Bong-Woo, Cohen, Oded, Seo, Eunyoung, Kim, Seungill, Kim, Ki-Tae, Kim, June-Sik, Lee, Hye-Young, Shin, Chanseok, Reeves, Gregory, Jung, Kyongyong, Ashrafi, Hamid, Kim, Yong-Min, Choi, Jaeyoung, Huh, Jin Hoe, Paran, Ilan, Choi, Doil, Park, Jeong Mee, Lee, Je Min, Park, Junhyung, Seo, Young Sam, Kang, Won-Hee, Lim, Jae Yun, van Deynze, Allen, Jo, Yeong Deuk, Park, Beom-Seok

2014

Overview

Doil Choi and colleagues report the genome sequence of the hot pepper, Capsicum annuum , as well as the resequencing of two cultivated peppers and a wild species, Capsicum chinense . Comparative genomic analysis across Solanaceae provides insights into genome expansion, pungency, ripening and disease resistance in hot peppers. Hot pepper ( Capsicum annuum ), one of the oldest domesticated crops in the Americas, is the most widely grown spice crop in the world. We report whole-genome sequencing and assembly of the hot pepper (Mexican landrace of Capsicum annuum cv. CM334) at 186.6× coverage. We also report resequencing of two cultivated peppers and de novo sequencing of the wild species Capsicum chinense . The genome size of the hot pepper was approximately fourfold larger than that of its close relative tomato, and the genome showed an accumulation of Gypsy and Caulimoviridae family elements. Integrative genomic and transcriptomic analyses suggested that change in gene expression and neofunctionalization of capsaicin synthase have shaped capsaicinoid biosynthesis. We found differential molecular patterns of ripening regulators and ethylene synthesis in hot pepper and tomato. The reference genome will serve as a platform for improving the nutritional and medicinal values of Capsicum species.

• Publisher: Nature Publishing Group US,Nature Publishing Group
• Subject: 38 / 45 / 45/23 / 45/91 / 631/208/212 / 631/208/2491 / 631/208/514 / Agriculture / Animal Genetics and Genomics / Biomedicine / Biosynthesis / Cancer Research / Capsaicin - metabolism / Capsicum - genetics / Capsicum - growth & development / Capsicum - metabolism / Capsicum annuum / Capsicum chinense / Cooking / Cultivars / Evolution, Molecular / Gene Expression Regulation, Developmental / Gene Expression Regulation, Plant / Gene Function / Genetic aspects / Genetic research / Genetic Variation / Genome Size / Genome, Plant / Genomes / Genomics / Human Genetics / Lycopersicon esculentum / Lycopersicon esculentum - genetics / Metabolic Networks and Pathways - genetics / Molecular Sequence Data / Multigene Family / Peppers / Plant genetics / RNA, Plant - genetics / Scholarships & fellowships / Species Specificity / Tomatoes / Vegetables