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A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung
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A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung
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Journal Article
A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung
2023
Overview
We present an in-depth single-cell atlas of in vitro multiculture systems on human primary airway epithelium derived from normal and diseased lungs of 27 individual donors. Our large-scale single-cell profiling identified new cell states and differentiation trajectories of rare airway epithelial cell types in human distal lungs. By integrating single-cell datasets of human lung tissues, we discovered immune-primed subsets enriched in lungs and organoids derived from patients with chronic respiratory disease. To demonstrate the full potential of our platform, we further illustrate transcriptomic responses to various respiratory virus infections in vitro airway models. Our work constitutes a single-cell roadmap for the cellular and molecular characteristics of human primary lung cells in vitro and their relevance to human tissues in vivo.
Organ models: Checking a match with human lung tissue
A new atlas of single cell profiles from human lungs provides a powerful platform for comparing immune responses in vitro and in vivo. The COVID-19 pandemic highlighted the usefulness of growing mini human organ cultures, called organoids, to understand the pathogenesis of diseases, especially in the epithelial cells that line the lungs. However, it is vital to confirm that these model systems accurately replicate the gene expression of real tissues. Jong-Il Kim at Seoul National University and co-workers established a biobank of 83 organoid lines derived from 27 healthy and diseased donor lungs, and verified that their transcriptomes—single cell mRNA expression profiles – were similar to those of real human lung tissues. This enabled them to map the differentiation of resident stem cells into rare airway types, and discover distinct subsets of cells primed for immunity.
Publisher
Nature Publishing Group UK,Springer Nature B.V,Nature Publishing Group
Subject
/ 13/100
/ 13/106
/ 14
/ 45
/ 64
/ Biomedical and Life Sciences
/ Cell Differentiation - physiology
/ COVID-19
/ Epithelial Cells - metabolism
/ Humans
/ Lung
/ Lungs
