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Effects of procyanidin on cardiomyocyte apoptosis after myocardial ischemia reperfusion in rats
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Effects of procyanidin on cardiomyocyte apoptosis after myocardial ischemia reperfusion in rats
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Effects of procyanidin on cardiomyocyte apoptosis after myocardial ischemia reperfusion in rats
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Journal Article
Effects of procyanidin on cardiomyocyte apoptosis after myocardial ischemia reperfusion in rats
2018
Overview
Background
This study is aimed at investigating the effects of procyanidin on cardiomyocyte apoptosis of myocardial ischemia/reperfusion (I/R) injury in rats.
Methods
Sprague-Dawley rats were randomly assigned into four groups: control group (normal saline), ischemic group (normal saline), procyanidin low-dose group (procyanidin 50 mg/kg/day) and procyanidin high-dose group (procyanidin 100 mg/kg/day) by intragastric administration for 2 weeks. After last administration, myocardial I/R model was induced by ligating left anterior descending artery for 30 min followed by 120 min of perfusion. The activity of serum creatine kinase mb isoenzyme (CK-MB) was detected after experiment. The content of reactive oxygen species (ROS) was determined by ROS fluorescent probe dihydroethidium; the expressions of p53, Caspase-9, Caspase-3, Bcl-2 and Bax were determined by western blotting; myocardial apoptosis was measured by the method of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.
Results
Compared with control group, the contents of serum CK-MB, ROS, the expressions of p53, Caspase-9, Caspase-3 and Bax increased significantly in ischemic group, the Bcl-2 expression, Bcl-2/Bax ratio decreased and the cardiomyocyte apoptosis index increased (
p
< 0.05); compared with ischemic group, the content of CK-MB, ROS, the expressions of p53, Caspase-9, Caspase-3 and Bax decreased, the Bcl-2 expression, Bcl-2/Bax ratio increased and the cardiomyocyte apoptosis index decreased in procyanidin group (
p
< 0.05).
Conclusions
Procyanidin can reduce cardiomyocyte apoptosis after I/R. This beneficial effect is partially dependant on decreased ROS, p53, Caspase-9, Caspase-3 and Bax, as well as increased Bcl-2 and Bcl-2/Bax ratio.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Animals
/ Apoptosis Regulatory Proteins - metabolism
/ Creatine Kinase, MB Form - blood
/ Ischemia
/ Male
/ Medicine
/ Myocardial Reperfusion Injury - metabolism
/ Myocardial Reperfusion Injury - pathology
/ Myocardial Reperfusion Injury - prevention & control
/ Myocytes, Cardiac - drug effects
/ Myocytes, Cardiac - metabolism
/ Myocytes, Cardiac - pathology
/ Oxidative Stress - drug effects
/ Proanthocyanidins - pharmacology
