Journal Article

New data analysis for BioSAXS at the ESRF

Oscarsson, Marcus, Pernot, Petra, Florial, Jean-Baptiste, Kieffer, Jérôme, Brennich, Martha, De Maria Antolinos, Alejandro, Tully, Mark

2022

Overview

The second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a new wiggler source and a larger detector. All analysis software has been rewritten to cope with the increased data flux and continues to provide beamline users with reduced and pre‐processed data in real time. This article describes FreeSAS, an open‐source collection of various small‐angle scattering analysis algorithms needed to reduce and analyze BioSAXS data, and Dahu, the tool used to interface data analysis with beamline control. It further presents the data‐processing pipelines for the different data acquisitions modes of the beamline, using either a sample changer for individual homogeneous samples or an inline size‐exclusion chromatography setup. A detailed presentation of the automatic data analysis pipelines for the BioSAXS beamline at the European synchrotron.

• Publisher: International Union of Crystallography,John Wiley & Sons, Inc
• Subject: Algorithms / automation / biological small-angle x-ray scattering / BioSAXS / Chromatography, Gel / Computer Programs / Data acquisition / Data Analysis / high brilliance / high-throughput saxs / online data analysis / online purification / Physics / proteins / Scattering, Small Angle / size-exclusion chromatography / Software / solution scattering / structural biology / Synchrotrons