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Quantitative Live Imaging Reveals Phase Dependency of PDAC Patient‐Derived Organoids on ERK and AMPK Activity
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Quantitative Live Imaging Reveals Phase Dependency of PDAC Patient‐Derived Organoids on ERK and AMPK Activity
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Journal Article
Quantitative Live Imaging Reveals Phase Dependency of PDAC Patient‐Derived Organoids on ERK and AMPK Activity
2025
Overview
Patient‐derived organoids represent a novel platform to recapitulate the cancer cells in the patient tissue. While cancer heterogeneity has been extensively studied by a number of omics approaches, little is known about the spatiotemporal kinase activity dynamics. Here we applied a live imaging approach to organoids derived from 10 pancreatic ductal adenocarcinoma (PDAC) patients to comprehensively understand their heterogeneous growth potential and drug responses. By automated wide‐area image acquisitions and analyses, the PDAC cells were non‐selectively observed to evaluate their heterogeneous growth patterns. We monitored single‐cell ERK and AMPK activities to relate cellular dynamics to molecular dynamics. Furthermore, we evaluated two anti‐cancer drugs, a MEK inhibitor, PD0325901, and an autophagy inhibitor, hydroxychloroquine (HCQ), by our analysis platform. Our analyses revealed a phase‐dependent regulation of PDAC organoid growth, where ERK activity is necessary for the early phase and AMPK activity is necessary for the late stage of organoid growth. Consistently, we found PD0325901 and HCQ target distinct organoid populations, revealing their combination is widely effective to the heterogeneous cancer cell population in a range of PDAC patient‐derived organoid lines. Together, our live imaging quantitatively characterized the growth and drug sensitivity of human PDAC organoids at multiple levels: in single cells, single organoids, and individual patients. This study will pave the way for understanding the cancer heterogeneity and promote the development of new drugs that eradicate intractable cancer. We applied a comprehensive live imaging approach to organoids derived from 10 pancreatic ductal adenocarcinoma (PDAC) patients. By automated image acquisitions and analyses, we found ERK and AMPK activity are important in the early and late phases of organoid growth, respectively.
Publisher
John Wiley & Sons, Inc,John Wiley and Sons Inc
Subject
/ AMP-Activated Protein Kinases - metabolism
/ AMPK
/ Antineoplastic Agents - pharmacology
/ Cancer
/ Carcinoma, Pancreatic Ductal - diagnostic imaging
/ Carcinoma, Pancreatic Ductal - drug therapy
/ Carcinoma, Pancreatic Ductal - metabolism
/ Carcinoma, Pancreatic Ductal - pathology
/ Cell Proliferation - drug effects
/ Diphenylamine - analogs & derivatives
/ Diphenylamine - pharmacology
/ ERK
/ Extracellular signal-regulated kinase
/ Extracellular Signal-Regulated MAP Kinases - metabolism
/ Humans
/ Hydroxychloroquine - pharmacology
/ Kinases
/ Lasers
/ MAP Kinase Signaling System - drug effects
/ Original
/ Pancreatic Neoplasms - diagnostic imaging
/ Pancreatic Neoplasms - drug therapy
/ Pancreatic Neoplasms - metabolism
/ Pancreatic Neoplasms - pathology
/ Plasmids
