Asset Details
Do you wish to reserve the book?
Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys
Do you wish to request the book?
Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys
2010
Overview
Background
The highly pathogenic H5N1 is a major avian pathogen that crosses species barriers and seriously affects humans as well as some mammals. It mutates in an intensified manner and is considered a potential candidate for the possible next pandemic with all the catastrophic consequences.
Methods
Nasal swabs were collected from donkeys suffered from respiratory distress. The virus was isolated from the pooled nasal swabs in specific pathogen free embryonated chicken eggs (SPF-ECE). Reverse transcriptase polymerase chain reaction (RT-PCR) and sequencing of both haemagglutingin and neuraminidase were performed. H5 seroconversion was screened using haemagglutination inhibition (HI) assay on 105 donkey serum samples.
Results
We demonstrated that H5N1 jumped from poultry to another mammalian host; donkeys. Phylogenetic analysis showed that the virus clustered within the lineage of H5N1 from Egypt, closely related to 2009 isolates. It harboured few genetic changes compared to the closely related viruses from avian and humans. The neuraminidase lacks oseltamivir resistant mutations. Interestingly, HI screening for antibodies to H5 haemagglutinins in donkeys revealed high exposure rate.
Conclusions
These findings extend the host range of the H5N1 influenza virus, possess implications for influenza virus epidemiology and highlight the need for the systematic surveillance of H5N1 in animals in the vicinity of backyard poultry units especially in endemic areas.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Animals
/ Biomedical and Life Sciences
/ Hemagglutinin Glycoproteins, Influenza Virus - genetics
/ Humans
/ Influenza A Virus, H5N1 Subtype - classification
/ Influenza A Virus, H5N1 Subtype - genetics
/ Influenza A Virus, H5N1 Subtype - isolation & purification
/ Influenza A Virus, H5N1 Subtype - pathogenicity
/ Orthomyxoviridae Infections - epidemiology
/ Orthomyxoviridae Infections - transmission
/ Orthomyxoviridae Infections - veterinary
/ Orthomyxoviridae Infections - virology
/ Respiratory Tract Infections - epidemiology
/ Respiratory Tract Infections - transmission
/ Respiratory Tract Infections - veterinary
/ Respiratory Tract Infections - virology
