Journal Article

The cyclin-dependent kinase inhibitor KRP6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in Arabidopsis

Verkest, Aurine, Universiteit Gent = Ghent University = Université de Gand (UGENT), Vieira, Paulo, Antonino de Souza Junior, Jose Dijair, de Veylder, Lieven, Institut Sophia Agrobiotech (ISA) ; Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Centre National de la Recherche Scientifique (CNRS), de Almeida Engler, Janice, van Isterdael, Gert, Inzé, Dirk, Glab, Nathalie, Flanders Institute for Biotechnology, Laboratório de Interação Molecular Planta-Praga ; Embrapa Recursos Genéticos e Biotecnologia = Embrapa Genetic Resources & Biotechnology (CENARGEN) ; Brazilian Agricultural Research Corporation = Empresa Brasileira de Pesquisa Agropecuária (Embrapa)-Brazilian Agricultural Research Corporation = Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Eeckhout, Dominique, van de Slijke, Eveline, de Clercq, Annelies, de Jaeger, Geert, Department of plant Biotechnology and Bioinformatics ; Universiteit Gent = Ghent University = Université de Gand (UGENT), Engler, Gi

2014

Overview

In Arabidopsis thaliana, seven cyclin-dependent kinase (CDK) inhibitors have been identified, designated interactors of CDKs or Kip-related proteins (KRPs). Here, the function of KRP6 was investigated during cell cycle progression in roots infected by plant-parasitic root-knot nematodes. Contrary to expectations, analysis of Meloidogyne incognita-induced galls of KRP6-overexpressing lines revealed a role for this particular KRP as an activator of the mitotic cell cycle. In accordance, KRP6-overexpressing suspension cultures displayed accelerated entry into mitosis, but delayed mitotic progression. Likewise, phenotypic analysis of cultured cells and nematode-induced giant cells revealed a failure in mitotic exit, with the appearance of multinucleated cells as a consequence. Strong KRP6 expression upon nematode infection and the phenotypic resemblance between KRP6 overexpression cell cultures and root-knot morphology point toward the involvement of KRP6 in the multinucleate and acytokinetic state of giant cells. Along these lines, the parasite might have evolved to manipulate plant KRP6 transcription to the benefit of gall establishment.

• Publisher: American Society of Plant Biologists (ASPB),CCSD,American Society of Plant Biologists
• Subject: Agricultural sciences / Arabidopsis / Cell culture techniques / Cell cycle / Cell lines / Cell nucleus / cyclin-dependent kinase / cytokinesis / genes / Giant cells / Life Sciences / Mitosis / Nematodes / Plant cells / Plant gall / plant parasitic nematodes / Plants
• ISBN: 0003418840000