Journal Article

Amplification-free Illumina sequencing-library preparation facilitates improved mapping and assembly of (G+C)-biased genomes

Ning, Zemin, Quail, Michael A, Berriman, Matthew, Sanders, Mandy J, Kozarewa, Iwanka, Turner, Daniel J

2009

Overview

The PCR step in the preparation of sequencing libraries for the Illumina Genome Analyzer can introduce coverage bias, especially in very (A+T)-rich genomes. By directly annealing template DNA to adapters with sequences needed for attachment in the flow cell, PCR can be omitted as cluster amplification in the flow cell enriches for fully ligated templates. Amplification artifacts introduced during library preparation for the Illumina Genome Analyzer increase the likelihood that an appreciable proportion of these sequences will be duplicates and cause an uneven distribution of read coverage across the targeted sequencing regions. As a consequence, these unfavorable features result in difficulties in genome assembly and variation analysis from the short reads, particularly when the sequences are from genomes with base compositions at the extremes of high or low G+C content. Here we present an amplification-free method of library preparation, in which the cluster amplification step, rather than the PCR, enriches for fully ligated template strands, reducing the incidence of duplicate sequences, improving read mapping and single nucleotide polymorphism calling and aiding de novo assembly. We illustrate this by generating and analyzing DNA sequences from extremely (G+C)-poor ( Plasmodium falciparum ), (G+C)-neutral ( Escherichia coli ) and (G+C)-rich ( Bordetella pertussis ) genomes.

• Publisher: Nature Publishing Group US,Nature Publishing Group
• Subject: Base Composition / Base Sequence / Bioinformatics / Biological Microscopy / Biological Techniques / Biomedical and Life Sciences / Biomedical Engineering/Biotechnology / Bordetella pertussis / Chromosome Mapping - methods / Deoxyribonucleic acid / DNA / DNA sequencing / E coli / Escherichia coli / Gene amplification / Gene Library / Genetic aspects / Genomics / Life Sciences / Malaria / Methods / Microbiology / Molecular Sequence Data / Nucleic Acid Amplification Techniques / Nucleotide sequencing / Plasmodium falciparum / Polymerase chain reaction / Polymorphism, Single Nucleotide - genetics / Proteomics / Research methodology / Sequence Analysis, DNA - methods