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坡柳种子对脱水干燥的生理生化响应
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坡柳种子对脱水干燥的生理生化响应
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Journal Article
坡柳种子对脱水干燥的生理生化响应
2011
Overview
为研究超干燥保存手段运用于坡柳种子的可行性，采用正交设计从种子含水量、包装方法、保存温度和预回湿方法4个因素来分析。用硅胶将种子脱水至1．12％～6．13％6个含水量梯度，结合不同处理密封保存1年，随后测定发芽率和一些生理生化指标，包括相对电导率（RC）、脯氨酸（Pro）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、可溶性总糖含量和脂肪酸组分。结果表明：种子含水量是对发芽率影响最显著的因素，较为适宜的脱水范围为4．28％～5．25％；超干种子发芽率与相对电导率和丙二醛含量呈显著负相关，与脯氨酸含量和3种抗氧化酶活性呈显著正相关；超干种子的油酸和d．亚麻酸含量比对照提高。坡柳种子保存过程中的劣变是由细胞膜损伤和脂质过氧化所引起，而适宜的脱水处理能使抗氧化酶活性增强，使不饱和脂肪酸的含量增加，从而维持种子生活力的稳定。
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