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Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy–treated macaques
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Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy–treated macaques
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Journal Article
Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy–treated macaques
2015
Overview
ImmunoPET/CT imaging using a labeled SIV-specific antibody can identify sites of viralinfection in SIV-infected macaques without the need for tissue biopsies, even if viral load in the blood is below the detection limit.
The detection of viral dynamics and localization in the context of controlled HIV infection remains a challenge and is limited to blood and biopsies. We developed a method to capture total-body simian immunodeficiency virus (SIV) replication using immunoPET (antibody-targeted positron emission tomography). The administration of a poly(ethylene glycol)-modified,
64
Cu-labeled SIV Gp120–specific antibody led to readily detectable signals in the gastrointestinal and respiratory tract, lymphoid tissues and reproductive organs of viremic monkeys. Viral signals were reduced in aviremic antiretroviral-treated monkeys but detectable in colon, select lymph nodes, small bowel, nasal turbinates, the genital tract and lung. In elite controllers, virus was detected primarily in foci in the small bowel, select lymphoid areas and the male reproductive tract, as confirmed by quantitative reverse-transcription PCR (qRT-PCR) and immunohistochemistry. This real-time,
in vivo
viral imaging method has broad applications to the study of immunodeficiency virus pathogenesis, drug and vaccine development, and the potential for clinical translation.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ 14/10
/ 59/78
/ Adenine - analogs & derivatives
/ Animals
/ Anti-Retroviral Agents - therapeutic use
/ Biomedical Engineering/Biotechnology
/ Biopsy
/ Blood
/ Deoxycytidine - analogs & derivatives
/ Deoxycytidine - therapeutic use
/ Macaca
/ Male
/ Membrane Glycoproteins - metabolism
/ Methods
/ Monkeys
/ Naphthyridines - therapeutic use
/ Nose
/ Organophosphonates - therapeutic use
/ Positron emission tomography
/ Positron-Emission Tomography - methods
/ Reverse Transcriptase Polymerase Chain Reaction
/ Simian Immunodeficiency Virus
/ Vaccines
/ Viral Envelope Proteins - metabolism
/ Viremia
/ Viruses
