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杨树类锌指基因ZFL的功能分析
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杨树类锌指基因ZFL的功能分析
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Journal Article
杨树类锌指基因ZFL的功能分析
2013
Overview
以毛果杨叶片cDNA为模板，采用PCR技术分离出杨树ZFL基因，序列分析结果表明该基因序列开放读码框315bp，共编码104个氨基酸，推导的蛋白质分子量为11．202kDa，理论等电点9．83，命名为PtrZFL。对ZFL基因进行实时荧光定量PCR表达分析，结果表明：甘露醇、NaCl、H2O2、ABA、低温胁迫都能诱导PtrZFL基因的表达，且PtrZFL表达量在ABA处理3h时达到最高，然后随处理时间延长而逐渐降低；低温（4℃）胁迫在6h后能显著诱导PtrZFL基因表达，并随着低温处理能一直保持较高水平的表达。根据毛果杨基因组信息设计引物，获得了PtrZFL基因上游1000bp的启动子序列，序列分析结果表明该启动子包含有多个与胁迫相关的元件，如抗冻、缺水、抗寒、脱落酸响应元件ABRE、MYB和WRKY。GUS活性检测发现，该启动子在转基因拟南芥整株中都有表达，但存根部和成熟叶片中表达较强，其他位置表达微弱。
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