Journal Article

Cytoprotective activities of milk thistle seed oil used in traditional tunisian medicine on 7-ketocholesterol and 24s-hydroxycholesterol-induced toxicity on 158n murine oligodendrocytes

Institut préparatoire aux Etudes Scientifiques et Techniques, Brétillon, Lionel, High Institute of Food Industries ; Partenaires INRAE, Vejux, Anne, Prost, Michel, Laboratoire Bio-PeroxIL. Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique (Bio-PeroxIL) ; Université de Bourgogne (UB), Université de Carthage (Tunisie) = University of Carthage (UCAR), Meddeb, Wiem, Lizard, Gérard, جامعة المنستير - Université de Monastir - University of Monastir (UM), جامعة سوسة = Université de Sousse = University of Sousse (USO), Nury, Thomas, Laboratoire de Recherche Appliquée Spiral [Bourgogne] (LARA SPIRAL), Zarrouk, Amira, Laboratoire Bio-PeroxIL. Biochimie du peroxysome, inflammation et métabolisme lipidique [Dijon] (BIO-PEROXIL) ; Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Rezig, Leila, Faculté des Sciences de Bizerte [Université de Carthage] ; Université de Carthage (Tunisie) = University of Carthage (UCAR), Mejri, M

2018

Overview

The Asteraceae family is economically very important, because many of these plants are grown mainly for their food value, such as lettuce (Lactuca), chicory (Cichorium), and sunflower (Heliantus aminus). One of the typical properties of this family, which includes milk thistle (Sylibum marianum), is the richness of the oil in various compounds (flavonoids, alkaloids, tocopherols, and unsaturated fatty acids). Currently, and for the coming decades, age-related diseases, including neurodegenerative diseases, are a major public health problem. Preventing their appearance or opposing their evolution is a major objective. In this context, the cytoprotective activities of milk thistle seed oil produced in Tunisia were studied on the 158N model using 7-ketocholesterol (7KC) and 24S-hydroxycholesterol (24S) as cytotoxic agents. 7KC and 24S were used because they can be increased in the brain and body fluids of patients with major age-related neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases. In order to evaluate the cytoprotective properties of milk thistle seed oil, complementary techniques of microscopy, flow cytometry, and biochemistry were used. The chemical composition of milk thistle seed oil has also been determined by various chromatography techniques. Milk thistle seed oils from different area of Tunisia are rich in tocopherols and are strongly antioxidant according to various biochemical tests (KRL (Kit Radicaux Libres), FRAP (Ferric Reducing Antioxidant Power), and DPPH (2,2-diphenyl-1-picrylhydrazyl)). The main fatty acids are linoleic acid (C18:2 n-6) and oleic acid (C18:1 n-9). The main polyphenols identified are homovanillic acid, p-coumaric acid, quercetin, and apigenin, with a predominance of vanillic acid. On 158N cells, milk thistle seed oil attenuates the cytotoxicity of 7KC and 24S including: loss of cell adhesion, increased plasma membrane permeability, mitochondrial dysfunction, overproduction of reactive oxygen species, induction of apoptosis, and autophagy. The attenuation of the cytotoxicity of 7KC and 24S observed with the milk thistle seed oil is in the order of that observed with alpha-tocopherol used as a positive control. In the presence of nigella seed oil, considered potentially cytotoxic, no cytoprotective effects were observed. Given the chemical characteristics, antioxidant properties, and cytoprotective activities of milk thistle seed oil, our results highlight the potential benefit of this oil for human health.

• Publisher: MDPI,CCSD,MDPI AG
• Subject: 158N murine oligodentrocytes / 24S-hydroxycholesterol / 7-ketocholesterol / cytoprotection / Food and Nutrition / Human health and pathology / Life Sciences / milk thistle seed oil / Silybum marianum
• ISBN: 0004402074000