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Hexosamine biosynthesis disruption impairs GPI production and arrests Plasmodium falciparum growth at schizont stages
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Hexosamine biosynthesis disruption impairs GPI production and arrests Plasmodium falciparum growth at schizont stages
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Journal Article
Hexosamine biosynthesis disruption impairs GPI production and arrests Plasmodium falciparum growth at schizont stages
2025
Overview
UDP-N-acetylglucosamine (UDP-GlcNAc) is a crucial sugar nucleotide for glycan synthesis in eukaryotes. In the malaria parasite Plasmodium falciparum , UDP-GlcNAc is synthesized via the hexosamine biosynthetic pathway (HBP) and is essential for glycosylphosphatidylinositol (GPI) anchor production, the most prominent form of protein glycosylation in the parasite. In this study, we explore a conditional knockout of glucosamine-6-phosphate N-acetyltransferase ( Pf GNA1), a key HBP enzyme. Pf GNA1 depletion led to significant disruptions in HBP metabolites, impairing GPI biosynthesis and causing mislocalization of the merozoite surface protein 1 (MSP1), the most abundant GPI-anchored protein in the parasite. Furthermore, parasites were arrested at the schizont stage, exhibiting severe segmentation defects and an incomplete rupture of the parasitophorous vacuole membrane (PVM), preventing egress from host red blood cells. Our findings demonstrate the critical role of HBP and GPI biosynthesis in P. falciparum asexual blood stage development and underscore the potential of targeting these pathways as a therapeutic strategy against malaria.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Animals
/ Arrests
/ Control
/ Enzymes
/ Glucosamine 6-Phosphate N-Acetyltransferase - genetics
/ Glucosamine 6-Phosphate N-Acetyltransferase - metabolism
/ Glycan
/ Glycosylphosphatidylinositol
/ Glycosylphosphatidylinositols - biosynthesis
/ Glycosylphosphatidylinositols - metabolism
/ Growth
/ Humans
/ Malaria
/ Malaria, Falciparum - metabolism
/ Malaria, Falciparum - parasitology
/ Plasmodium falciparum - genetics
/ Plasmodium falciparum - growth & development
/ Plasmodium falciparum - metabolism
/ Proteins
/ Protozoan Proteins - genetics
/ Protozoan Proteins - metabolism
