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A comparison of methods for excluding light from stems to evaluate stem photosynthesis
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A comparison of methods for excluding light from stems to evaluate stem photosynthesis
A comparison of methods for excluding light from stems to evaluate stem photosynthesis
Journal Article

A comparison of methods for excluding light from stems to evaluate stem photosynthesis

2023
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Overview
Premise A comparison of methods using different materials to exclude light from stems to prevent stem CO2 exchange (i.e., photosynthesis), without affecting stem conductance to water vapor, surface temperature, and relative humidity, was conducted on stems of avocado trees in California. Methods and Results The experiment featured three materials: aluminum foil, paper‐based wrap, and mineral‐based paint. We examined stem CO2 exchange with and without the light exclusion treatments. We also examined stem surface temperature, relative humidity, and photosynthetic active radiation (PAR) under the cover materials. All materials reduced PAR and stem CO2 exchange. However, aluminum foil reduced stem surface temperature and increased relative humidity. Conclusions Methods used to study stem CO2 exchange through light exclusion have historically relied on methods that may induce experimental artifacts. Among the methods tested here, mineral‐based paint effectively reduced PAR without affecting stem surface temperature and relative humidity around the stem. Resumen Premisa Una comparación de diferentes métodos utilizando distintos materiales para bloquear la luz de los tallos y así reducir el intercambio de CO2 (fotosíntesis) sin afectar la conductancia del tallo al vapor de agua, su temperatura superficial y la humedad relativa fue llevado a cabo en tallos de árboles de aguacate en California. Metodología y resultados El experimento se llevó a cabo utilizando tres materiales: papel de aluminio, papel para envoltura y pintura a base de minerales. Se examinó el intercambio de CO2 de los tallos con y sin los materiales de bloqueo de la luz. También se examinó la temperatura de la superficie del tallo, la humedad relativa y la radiación fotosintéticamente activa (PAR por sus siglas en inglés) debajo de los materiales usados para bloquear la luz. Todos los materiales redujeron PAR y el intercambio de CO2 del tallo. Sin embargo, el papel aluminio redujo también la temperatura de la superficie del tallo y aumento la humedad relativa. Conclusiones Los métodos utilizados para estudiar el intercambio de CO2 de los tallos con el ambiente a través del bloqueo de la luz han sido métodos que pueden generar alteraciones no deseadas. Entre los métodos evaluados aquí, la pintura de base mineral fue efectiva reduciendo PAR sin alterar la temperatura superficial del tallo ni la humedad relativa alrededor de este.