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A xylose-inducible expression system for Lactococcus lactis
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A xylose-inducible expression system for Lactococcus lactis
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Journal Article
A xylose-inducible expression system for Lactococcus lactis
2004
Overview
A new controlled production system to target heterologous proteins to cytoplasm or extracellular medium is described for
Lactococcus lactis NCDO2118. It is based on the use of a xylose-inducible lactococcal promoter, P
xylT
. The capacities of this system to produce cytoplasmic and secreted proteins were tested using the
Staphylococcus aureus nuclease gene (
nuc) fused or not to the lactococcal Usp45 signal peptide. Xylose-inducible
nuc expression is tightly controlled and resulted in high-level and long-term protein production, and correct targeting either to the cytoplasm or to the extracellular medium. Furthermore, this expression system is versatile and can be switched on or off easily by adding either xylose or glucose, respectively. These results confirm the potential of this expression system as an alternative and useful tool for the production of proteins of interest in
L. lactis.
Publisher
Elsevier B.V,Blackwell Publishing Ltd,Blackwell,Oxford University Press
Subject
/ Biological and medical sciences
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation, Bacterial - drug effects
/ Genetics
/ Growth, nutrition, cell differenciation
/ Lactococcus lactis - genetics
/ Lactococcus lactis - metabolism
/ Micrococcal Nuclease - metabolism
/ Nuclease
/ Proteins
/ Recombinant Fusion Proteins - analysis
/ Xylose
/ Yeast
