Journal Article

白蜡虫蜡酯合酶基因cDNA全长克隆及原核表达

刘博文 王雪庆 孙涛 亓倩 于淑惠 杨璞 陈晓鸣

2016

Overview

S899.1; Objective]To obtain the full length cDNA sequence of the wax synthase (ws)gene of the white wax in-sect,Ericerus pela Chavannes,and heterologously express the enzyme in Escherichia coli.[Method]The 3′and 5′ends of the ws gene were obtained separately by using RACE,and the prokaryotic expression vector was constructed after the analysis of the full length cDNA sequence of the ws gene.Finally,the expression of WS in Ericerus coli BL21 was induced by IPTG.[Result]Sequence analysis showed that,the full-length cDNA of the ws gene was 1 518 bp,which included the 5′-UTR (untranslated region)with 94 bp,3′-UTR with 68 bp,and the open reading frame with 1 356 bp.The gene was deduced to encode 452 amino acid residues with the putative protein molecular weight of 51.8 kDa,and the theoretical isoelectric point of 6.35.Western Blot result showed that,the WS enzyme was expressed successfully in Ericerus coli.[Conclusion]In this study,the full length cDNA of the ws gene of Ericerus pela was obtained,and this enzyme w

• Publisher: 中国林业科学研究院资源昆虫研究所，国家林业局资源昆虫培育与利用重点实验室，云南 昆明 650224
• Subject: cDNA全长基因 / 原核表达 / 白蜡虫 / 蜡酯合酶