Asset Details
Do you wish to reserve the book?
LRRC3B在非小细胞肺癌中下调并与肺癌细胞增殖和侵袭能力相关
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
LRRC3B在非小细胞肺癌中下调并与肺癌细胞增殖和侵袭能力相关
Do you wish to request the book?
LRRC3B在非小细胞肺癌中下调并与肺癌细胞增殖和侵袭能力相关
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
LRRC3B在非小细胞肺癌中下调并与肺癌细胞增殖和侵袭能力相关
2016
Overview
背景与目的已有的研究表明：在许多恶性肿瘤细胞中，LRRC3B表达显著下调，被视为肿瘤抑制蛋白。然而，在非小细胞肺癌中它的表达模式和生物学作用缺乏研究。人类癌症微阵列的研究显示LR-RC3B在乳腺癌和结肠直肠癌表达下调，提示LRRC3B参与致癌作用。本研究的目的是研究LRRC3B在非小细胞肺癌中的必到状态及其与肺癌增殖、侵袭和细胞周期间的相关性，探讨LRRC3B在调控肺癌细胞增殖、侵袭及细胞周期中的作用。方法应用Western blot和Realtime RT-PCR检测LRRC3B在几株肺癌细胞系中的mRNA和蛋白表达水平。应用MTT法检测对转染LRRC3B的A549和H460细胞系细胞增殖能力变化，应用集落形成实验以及细胞侵袭实验研究LRRC3B对细胞增殖和侵袭以及细胞周期进程的作用。肺癌细胞系H3255中转染LR-RC3B siRAN验证LRRC3B对细胞的增殖以及侵袭能力和对细胞周期进程的影响。结果与正常NHBE细胞系相比，NSCLC细胞系中LRRC3B蛋白表达量显著下调，特别是H460、H358、HCC827以及A549。A549和H460细胞系转染LRRC3B后，细胞增殖和侵袭能力受到抑制。LRRC3B抑制细胞周期进程，并下调cyclin D1和MMP9的表达。H3255细胞中敲除LRRC3B，细胞增殖和侵袭能力显著增强，同时与细胞周期及侵袭能力相关的蛋白cyclin D1和MMP9表达略微上调。结论 LRRC3B在肺癌细胞系中表达下调，而上调LRRC3B则能够抑制肺癌细胞增殖和侵袭能力，并抑制细胞周期进程，可能是未来肺癌治疗的一个新靶点。
We currently cannot retrieve any items related to this title. Kindly check back at a later time.