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杨树ASE蛋白的生化功能研究
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杨树ASE蛋白的生化功能研究
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Journal Article
杨树ASE蛋白的生化功能研究
2016
Overview
[目的]谷氨酰胺磷酸核糖焦磷酸转酰胺酶（ASE）在植物嘌呤合成中发挥着关键作用,本研究的目的是详细揭示杨树ASE蛋白的生化功能特性。[方法]通过半定量RT-PCR、蛋白质亚细胞定位以及测定纯化蛋白的催化活性等方法来研究其在体外的生化功能,进一步通过互补拟南芥缺失突变体（atase2）来研究杨树ASE基因的体内生物学功能。[结果]从杨树基因组中鉴别出2个ASE基因,序列分析发现它们之间有很高的蛋白质序列相似性。半定量RT-PCR分析发现两个杨树ASE基因在根、茎、叶和芽中均表达。蛋白质亚细胞定位分析发现杨树ASE蛋白均定位在叶绿体中,而且两个杨树ASE蛋白均能完全互补拟南芥atase2突变体的表型。酶学性质分析表明,杨树两个ASE蛋白均能催化5-磷酸核糖-α-焦磷酸生成5-磷酸核糖-β-胺。[结论]本研究预示着两个杨树ASE蛋白在嘌呤合成中均发挥着重要作用。
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