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Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
by
Kearns, Anne
, Hawes, Chris
, Sparkes, Imogen A
, Runions, John
in
Analytical Chemistry
/ Binding proteins
/ Biological Techniques
/ Biomedical and Life Sciences
/ Cell culture
/ Cloning, Molecular - methods
/ Computational Biology/Bioinformatics
/ Culture techniques
/ Fluorescence
/ Gene Expression
/ Gene Transfer Techniques
/ Green Fluorescent Proteins - metabolism
/ Leaves
/ Life Sciences
/ Methods
/ Microarrays
/ Nicotiana - genetics
/ Nicotiana - metabolism
/ Organic Chemistry
/ Physiological aspects
/ Plants
/ Proteins
/ protocol
/ Recombinant Fusion Proteins - metabolism
/ Rhizobium - genetics
/ Tissue Culture Techniques
/ Tobacco
2006
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Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
by
Kearns, Anne
, Hawes, Chris
, Sparkes, Imogen A
, Runions, John
in
Analytical Chemistry
/ Binding proteins
/ Biological Techniques
/ Biomedical and Life Sciences
/ Cell culture
/ Cloning, Molecular - methods
/ Computational Biology/Bioinformatics
/ Culture techniques
/ Fluorescence
/ Gene Expression
/ Gene Transfer Techniques
/ Green Fluorescent Proteins - metabolism
/ Leaves
/ Life Sciences
/ Methods
/ Microarrays
/ Nicotiana - genetics
/ Nicotiana - metabolism
/ Organic Chemistry
/ Physiological aspects
/ Plants
/ Proteins
/ protocol
/ Recombinant Fusion Proteins - metabolism
/ Rhizobium - genetics
/ Tissue Culture Techniques
/ Tobacco
2006
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Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
by
Kearns, Anne
, Hawes, Chris
, Sparkes, Imogen A
, Runions, John
in
Analytical Chemistry
/ Binding proteins
/ Biological Techniques
/ Biomedical and Life Sciences
/ Cell culture
/ Cloning, Molecular - methods
/ Computational Biology/Bioinformatics
/ Culture techniques
/ Fluorescence
/ Gene Expression
/ Gene Transfer Techniques
/ Green Fluorescent Proteins - metabolism
/ Leaves
/ Life Sciences
/ Methods
/ Microarrays
/ Nicotiana - genetics
/ Nicotiana - metabolism
/ Organic Chemistry
/ Physiological aspects
/ Plants
/ Proteins
/ protocol
/ Recombinant Fusion Proteins - metabolism
/ Rhizobium - genetics
/ Tissue Culture Techniques
/ Tobacco
2006
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Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Journal Article
Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
2006
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Overview
Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in cell biology. The protocol presented here has underpinned much of the
in vivo
results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal cells is a relatively fast technique to assess expression of genes of interest. These cells can be used to generate stable plant lines using a more time-consuming, cell culture technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.
Publisher
Nature Publishing Group UK,Nature Publishing Group
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