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穗花杉的转录组测序及其转录组特性分析
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穗花杉的转录组测序及其转录组特性分析
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Journal Article
穗花杉的转录组测序及其转录组特性分析
2017
Overview
[目的]通过对红豆杉科穗花杉属的穗花杉进行转录组测序，为穗花杉的萜类合成途径和分类学研究提供支持。[方法]利用HiSeq2500技术对穗花杉的茎叶进行转录组测序。[结果]穗花杉转录组测序共得到8．14Gb的cleandata。从头组装共获得82884条unigene，其中有27495条unigene被注释。此外，在82884条unigene中共搜索到2827个SSR位点，单核苷酸重复SSR出现频率最高（60．1％），其次为三核苷酸重复SSR（25．4％）。在穗花杉unigene中挖掘出了1个牦牛儿基焦磷酸合成酶（GPS）、1个法尼烯基焦磷酸合成酶（FPS）和5个牦牛儿基牦牛儿基焦磷酸合成酶（GGPS）的同源基因，同时得到紫杉二烯合成酶（TS）同源基因13个。[结论]发现了20个与萜类合成有关的unigene和2827个SSR位点，为今后穗花杉萜类生物合成研究，特别是紫杉二烯合成酶编码基因的研究打下了基础，也为该物种系统分类地位及遗传多样性研究提供了新的遗传信息。
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