Asset Details
Do you wish to reserve the book?
Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor
Do you wish to request the book?
Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor
2012
Overview
Hepatitis B immunoglobulin （HBIG） is important in the management of hepatitis B virus （HBV） infection. Aiming to develop recombinant monoclona# antibodies as an alternative to HBIG, we report the successful identification of HBV surface antigen （HBsAg）-specific antibodies from a full-length human antibody library displayed on mammalian cell surface. Using total RNA of peripheral blood mononuclear cells of a natively immunized donor as template, the antibody repertoire was amplified. Combining four-way ligation and the FIp recombinase-mediated integration （FIp-ln） system, we constructed a mammalian cell-based, fully human, full-length antibody display library in which each cell displayed only one kind of antibody molecule. By screening the cell library using fluorescence-activated cell sorting （FACS）, eight cell clones that displayed HBsAg-specific antibodies on cell surfaces were identified. DNA sequence analysis of the antibody genes revealed three unique antibodies. FACS data indicated that fluorescent strength of expression （FSE）, fluorescent strength of binding （FSB） and relative binding ability （RBA） were all different among them. These results demonstrated that by using our antibody mammalian display and screening platform, we can successfully identify antigen-specific antibodies from an immunized full-length antibody library. Therefore, this platform is very useful for the development of therapeutic antibodies.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Antigens
/ Biomedical and Life Sciences
/ Hepatitis B - prevention & control
/ Hepatitis B Antibodies - genetics
/ Hepatitis B Antibodies - immunology
/ Hepatitis B Surface Antigens - immunology
/ Hepatitis B virus - genetics
/ Hepatitis B virus - immunology
/ Humans
/ Peripheral blood mononuclear cells
/ RNA
/ Vaccine
/ Viral Hepatitis Vaccines - genetics
/ Viral Hepatitis Vaccines - immunology
/ 乙肝表面抗原
/ 人类健康
/ 免疫球蛋白
/ 哺乳动物细胞
/ 抗体库
/ 显示
/ 特异性抗体
/ 鉴定
