Asset Details
Do you wish to reserve the book?
Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese Production
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese Production
Do you wish to request the book?
Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese Production
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese Production
2006
Overview
Biogenic amines are toxic substances that appear in foods and beverages as a result of AA decarboxylation. The enzyme histidine decarboxylase catalyzes the decarboxylation of histidine to histamine, the biogenic amine most frequently involved in food poisoning. The aim of the present work was to develop a real-time quantitative PCR assay for the direct detection and quantification of histamine-producing strains in milk and cheese. A set of primers was designed, based on the histidine decarboxylase gene sequence of different gram-positive bacteria. The results show the proposed procedure to be a rapid (total processing time <2h), specific and highly sensitive technique for detecting potential histamine-producing strains. Chromatographic methods (HPLC) verified the capacity of real-time quantitative PCR to correctly quantify histamine accumulation.
Publisher
Elsevier Inc,American Dairy Science Association,Am Dairy Sci Assoc
Subject
/ Animals
/ Biological and medical sciences
/ Cheese
/ cheeses
/ Chromatography, High Pressure Liquid
/ Chromatography, High Pressure Liquid - methods
/ Foodborne Diseases - microbiology
/ Foodborne Diseases - prevention & control
/ Fundamental and applied biological sciences. Psychology
/ genetics
/ Histidine Decarboxylase - genetics
/ Histidine Decarboxylase - metabolism
/ methods
/ Methods of analysis, processing and quality control, regulation, standards
/ Milk
/ Plasmids
/ Polymerase Chain Reaction - methods
/ real-time quantitative polymerase chain reaction
/ Streptococcaceae - classification
/ Streptococcaceae - enzymology
/ Streptococcaceae - isolation & purification
We currently cannot retrieve any items related to this title. Kindly check back at a later time.