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Engineering Pseudomonas putida KT2440 for chain length tailored free fatty acid and oleochemical production
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Engineering Pseudomonas putida KT2440 for chain length tailored free fatty acid and oleochemical production
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Journal Article
Engineering Pseudomonas putida KT2440 for chain length tailored free fatty acid and oleochemical production
2022
Overview
Despite advances in understanding the metabolism of
Pseudomonas putida
KT2440, a promising bacterial host for producing valuable chemicals from plant-derived feedstocks, a strain capable of producing free fatty acid-derived chemicals has not been developed. Guided by functional genomics, we engineered
P. putida
to produce medium- and long-chain free fatty acids (FFAs) to titers of up to 670 mg/L. Additionally, by taking advantage of the varying substrate preferences of paralogous native fatty acyl-CoA ligases, we employed a strategy to control FFA chain length that resulted in a
P. putida
strain specialized in producing medium-chain FFAs. Finally, we demonstrate the production of oleochemicals in these strains by synthesizing medium-chain fatty acid methyl esters, compounds useful as biodiesel blending agents, in various media including sorghum hydrolysate at titers greater than 300 mg/L. This work paves the road to produce high-value oleochemicals and biofuels from cheap feedstocks, such as plant biomass, using this host.
Pseudomonas putida
has been engineered to produce medium chain length oleochemicals, including medium chain length free fatty acids and their ester forms, compounds which may be useful as biodiesel blending agents.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Springer Nature,Nature Portfolio
