Asset Details
Do you wish to reserve the book?
GSTM1基因多态性及启动子甲基化在结肠癌细胞系中的研究
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
GSTM1基因多态性及启动子甲基化在结肠癌细胞系中的研究
Do you wish to request the book?
GSTM1基因多态性及启动子甲基化在结肠癌细胞系中的研究
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
GSTM1基因多态性及启动子甲基化在结肠癌细胞系中的研究
2014
Overview
目的研究参与内毒物代谢的重要酶谷胱甘肽-S-转移酶M1（glutathione-s—transferaseM1，GSTM1）在结肠癌细胞系中的基因表达状况与其遗传基因型及启动子甲基化的关系。方法采用多重PCR方法检测GSTM1基因型，甲基化特异PCR（MSP）方法检测GSTM1基因启动子甲基化状态；用5-aza-dC处理结肠癌细胞系，RT—PCR方法检测GSTM1基因转录水平。结果结肠癌细胞系HT29、SW480、SW1116为GSTM1非空白基因型，而HCT116、Lovo、Colo结肠癌细胞系为GSTMl空白基因型。MSP检测发现SW1116细胞中GSTMl基因主要呈非甲基化状态，其他5个细胞系中呈甲基化状态，而且基因表达缺失。经5-aza—dC处理后，HT29和SW480细胞的GSTM1基因表达复活，而HCT116、Lovo、Colo细胞中GSTM1仍无扩增。结论GSTM1基因转录受到遗传基因型和表观遗传启动子甲基化双重调控。
Publisher
上海交通大学医学院附属仁济医院消化科,上海市消化疾病研究所,上海,200001
Subject
We currently cannot retrieve any items related to this title. Kindly check back at a later time.