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bimodular nuclear localization signal assembled via an extended double-stranded RNA-binding domain acts as an RNA-sensing signal for transportin 1
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bimodular nuclear localization signal assembled via an extended double-stranded RNA-binding domain acts as an RNA-sensing signal for transportin 1
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bimodular nuclear localization signal assembled via an extended double-stranded RNA-binding domain acts as an RNA-sensing signal for transportin 1
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Journal Article
bimodular nuclear localization signal assembled via an extended double-stranded RNA-binding domain acts as an RNA-sensing signal for transportin 1
2014
Overview
The human RNA-editing enzyme adenosine deaminase acting on RNA (ADAR1) carries a unique nuclear localization signal (NLS) that overlaps one of its double-stranded RNA-binding domains (dsRBDs). This dsRBD-NLS is recognized by the nuclear import receptor transportin 1 (Trn1; also called karyopherin-β2) in an RNA-sensitive manner. Most Trn1 cargos bear a well-characterized proline-tyrosine-NLS, which is missing from the dsRBD-NLS. Here, we report the structure of the dsRBD-NLS, which reveals an unusual dsRBD fold extended by an additional N-terminal α-helix that brings the N- and C-terminal flanking regions in close proximity. We demonstrate experimentally that the atypical ADAR1-NLS is bimodular and is formed by the combination of the two flexible fragments flanking the folded domain. The intervening dsRBD acts only as an RNA-sensing scaffold, allowing the two NLS modules to be properly positioned for interacting with Trn1. We also provide a structural model showing how Trn1 can recognize the dsRBD-NLS and how dsRNA binding can interfere with Trn1 binding.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
Active Transport, Cell Nucleus
/ Adenosine Deaminase - chemistry
/ Adenosine Deaminase - genetics
/ Adenosine Deaminase - metabolism
/ beta Karyopherins - chemistry
/ beta Karyopherins - genetics
/ beta Karyopherins - metabolism
/ Enzymes
/ Humans
/ nuclear localization signals
/ Nuclear Localization Signals - chemistry
/ Nuclear Localization Signals - genetics
/ Nuclear Localization Signals - metabolism
/ Nuclear Magnetic Resonance, Biomolecular
/ Protein Interaction Domains and Motifs
/ Protein Structure, Secondary
/ Proteins
/ Recombinant Fusion Proteins - chemistry
/ Recombinant Fusion Proteins - genetics
/ Recombinant Fusion Proteins - metabolism
/ RNA
