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中药安迪粉针剂对离体肿瘤细胞的细胞毒作用
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Journal Article
中药安迪粉针剂对离体肿瘤细胞的细胞毒作用
2003
Overview
目的 观察以蟾酥和人参为主要配方的中药粉针剂安迪(Andi)对肿瘤细胞的药物毒性作用。方法 采用MTT法测定Andi对3株人肿瘤细胞的细胞生长曲线、细胞存活率(SR)和细胞倍增时间(TD)；观察Andi对ECA109细胞生长的时效关系。克隆计数法检测不同浓度Andi对ECA109细胞在含氧和乏氧状态下的生存分数(SF)，计算其乏氧细胞毒性比(HCR)和半数抑制浓度(IC50)。结果 ①Andi组和对照组对ECA109、HepG2、SGC7901细胞的TD分别为118．72、123．08、40．17h和46．12、40．17、19．67h；Andi组的SR分别为对照组的33．33％、50．54％、36．88％。②12．50、25．00、50．00μg·mL^-1Andi对ECA109细胞在有氧和乏氧环境中的SF分别为1．34、O．97、0．25和O．96、O．27、0．18。③Andi在乏氧环境中能抑制ECA109细胞的生长，其作用大小与Andi浓度及作用时间呈正相关。④在有氧和乏氧下Andi的IC50分别为39．60μg·mL^-1和23．50μg·mL^-1，HCR为2．02。结论 Andi对多种人肿瘤细胞具有乏氧细胞毒作用，其程度与Andi的浓度和作用时间呈正相关。
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