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siRNA沉默STAT3基因对肝癌细胞HepG2增殖的影响
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siRNA沉默STAT3基因对肝癌细胞HepG2增殖的影响
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Journal Article
siRNA沉默STAT3基因对肝癌细胞HepG2增殖的影响
2013
Overview
目的通过体外沉默信号传导及转录活化因子3（signal transducer and activator of transcription 3，STAT3）基因转录，探讨阻断JAK2／STAT3信号通路对肝癌细胞HepG2增殖能力的影响及其机制。方法siRNA体外沉默肝癌细胞株HepG2STAT3基因转录，检测细胞内STAT3mRNA和蛋白的表达变化证实基因沉默效果，MTT检测细胞增殖能力的变化，流式细胞仪检测细胞周期，RT—PCR检测JAK2／sTAT3信号通路下游分子survivin及caspase3表达的变化。结果阻断JAK2／STAT3信号通路后肝癌细胞株HepG2增殖能力下降，细胞分裂停滞于GOG1期，伴随survivin转录下降，caspase3转录上升。结论阻断JAK2／STAT3信号通路可抑制肝癌细胞增殖，可能同调控survivin及caspase3有关。
Publisher
西安交通大学医学院第一附属医院肝胆外科,陕西西安,710061%西安交通大学医院,陕西西安,710061
Subject
