Journal Article

RT-QuIC as ultrasensitive method for prion detection

Atarashi, Ryuichiro

2023

Overview

Real-time quaking-induced conversion (RT-QuIC) is a cell-free abnormal form of prion protein (PrP Sc ) amplification method using recombinant prion protein from Escherichia coli that can measure prion seeding activity in samples with high sensitivity. The advantages of this method are that it is much more sensitive than Western blotting, which is usually used to detect PrP Sc , and that prion seeding activity can be easily quantified by combining it with endpoint dilution of the sample, and that it can be amplified in most species and prion strains. A decade has passed since the development of RT-QuIC, and many studies have been reported that take advantage of its characteristics. In particular, its usefulness in the diagnosis of sporadic CJD has been clarified, and it is recommended to be one of the diagnostic criteria. Future challenges include the establishment of a method to differentiate prion strains and application of RT-QuIC to early diagnosis of prion diseases and determination of treatment efficacy.

• Publisher: Springer Berlin Heidelberg,Springer,Springer Nature B.V
• Subject: Analysis / Animals / Biomedical and Life Sciences / Biomedicine / Blotting, Western / Cattle / Cerebrospinal fluid / Creutzfeldt-Jakob disease / Creutzfeldt-Jakob Syndrome / Diagnosis / E coli / early diagnosis / Efficiency / Encephalopathy, Bovine Spongiform / Escherichia coli / genetic techniques and protocols / Human Genetics / Methods / Molecular Medicine / Prion Diseases - diagnosis / Prion protein / Prion Proteins / Prions / Protein seeding / Proteins / Proteomics / Recombinant Proteins / Review / Western blotting