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Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions
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Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions
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Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions
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Journal Article
Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions
2015
Overview
Background
Tight junctions (TJs) maintain the intestinal mucosal barrier, dysfunction of which plays a vital role in the pathophysiology of a variety of gastrointestinal disorders. Previously, we have shown that
L. reuteri
I5007 maintained the gut epithelial barrier in newborn piglets. Here we aimed to decipher the influence of
L. reuteri
I5007 on tight junction (TJ) protein expression both
in vivo
and
in vitro
.
Results
We found that
L. reuteri
I5007 significantly increased the protein abundance of intestinal epithelial claudin-1, occludin and zonula occluden-1 (ZO-1) in newborn piglets (orally administrated with 6 × 10
9
CFU of
L. reuteri
I5007 daily for 14 days).
In vitro
, treatment with
L. reuteri
I5007 alone maintained the transepithelial electrical resistance (TEER) of IPEC-J2 cells with time. In addition, IPEC-J2 cells were stimulated with 1 μg/mL lipopolysaccharide (LPS) for 1, 4, 8, 12 or 24 h, following pre-treatment with
L. reuteri
I5007 or its culture supernatant for 2 h. The results showed that LPS time-dependently induced (significantly after 4 or 8 h) the expression of TNF-α and IL-6, and decreased TJ proteins, which was reversed by pre-treatment of
L. reuteri
I5007 or its culture supernatant.
Conclusions
L. reuteri
I5007 had beneficial effects on the expression of TJ proteins in newborn piglets and the
in-vitro
results showed this strain had a positive effect on TEER of cells and inhibited the reduction of TJ proteins expression induced by LPS. These findings indicated
L. reuteri
I5007 may have potential roles in protection TJ proteins in TJ-deficient conditions.
Publisher
BioMed Central,BioMed Central Ltd
Subject
/ Animals
/ Biomedical and Life Sciences
/ Epithelial Cells - immunology
/ Epithelial Cells - metabolism
/ Epithelial Cells - microbiology
/ Lactobacillus reuteri - growth & development
/ Lactobacillus reuteri - immunology
/ Lipopolysaccharides - metabolism
/ Mitogens
/ Mycology
/ Swine
/ Tumor Necrosis Factor-alpha - metabolism
/ Virology
