Journal Article

Genome-wide identification and characterization of abiotic-stress responsive SOD (superoxide dismutase) gene family in Brassica juncea and B. rapa

Verma, Deepika, Lakhanpal, Neha, Singh, Kashmir

2019

Overview

Background Abiotic stresses like drought, heat, cold and salinity cause major productivity loss in the rapeseed-mustard crops ( Brassica ). Major efforts have been made in the past to identify genes that provide resistance against such stresses. Superoxide dismutase (SOD) proteins, member of the metallo-enzyme family play vital role in protecting plants against abiotic stresses. In the present study, genome-wide analysis of abiotic stress responsive SOD gene family has been done in B. juncea and B. rapa . Results A total of 29 and 18 SOD genes were identified in B. juncea and B. rapa respectively and chromosome location mapping indicated their wide distribution across genome. On the basis of domain composition, the SODs were phylogenetically classified into sub-groups which was also substantiated by the gene structure and sub-cellular locations of SOD proteins. Functional annotation of SODs was also done by Gene Ontology (GO) mapping and the result was corroborated by the identified cis -regulatory elements in the promoter region of SOD genes. Based on FPKM analysis of SRA data available for drought, heat and salt stress, we identified 14 and 10 abiotic stress responsive SOD genes in B. rapa and B. juncea respectively . The differential expression analysis under drought and heat stress of identified abiotic-stress responsive SOD genes was done through quantitative Real Time PCR. Conclusion We identified abiotic-stress responsive genes that could help in improving the plant tolerance against abiotic stresses. This was the first study to describe the genome-wide analysis of SOD gene family in B. rapa and B. juncea , and the results will help in laying basic ground for future work of cloning and functional validation of SOD genes during abiotic stresses leading to Brassica crop improvement.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: Abiotic stress / Animal Genetics and Genomics / B. rapa / Biomedical and Life Sciences / Brassica / Brassica juncea / Brassica rapa - enzymology / Brassica rapa - genetics / Brassica rapa - physiology / Chromosome Mapping / Droughts / Ecological stress / Environmental aspects / Enzymes / Gene Expression Profiling / Gene Expression Regulation, Enzymologic / Gene Expression Regulation, Plant / Gene family / Genes / Genetic aspects / Genetic research / Genome, Plant / Genome-wide analysis / Genomes / Genomics / Life Sciences / Microarrays / Microbial Genetics and Genomics / Multigene Family / Mustard Plant - enzymology / Mustard Plant - genetics / Mustard Plant - physiology / Phylogeny / Plant Genetics and Genomics / Plant genomics / Plant Proteins - genetics / Polymerase chain reaction / Production management / Proteins / Proteomics / Research Article / Salinity / Stress, Physiological / Superoxide dismutase / Superoxide Dismutase - genetics / Superoxides