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激光扫描共聚焦显微镜技术检测脑脊液单核细胞早期分泌抗原靶-6在结核性脑膜炎早期诊断中的应用
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激光扫描共聚焦显微镜技术检测脑脊液单核细胞早期分泌抗原靶-6在结核性脑膜炎早期诊断中的应用
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激光扫描共聚焦显微镜技术检测脑脊液单核细胞早期分泌抗原靶-6在结核性脑膜炎早期诊断中的应用
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Journal Article
激光扫描共聚焦显微镜技术检测脑脊液单核细胞早期分泌抗原靶-6在结核性脑膜炎早期诊断中的应用
2016
Overview
目的探讨激光扫描共聚焦显微镜（LSCM）技术检测脑脊液单核细胞早期分泌抗原靶-6（ESAT-6）在结核性脑膜炎早期诊断中的应用价值，为早期诊断寻找一种更特异性的检测方法。方法采用免疫荧光双标法检测ESAT-6抗原，LSCM观察脑脊液单核细胞着色情况（ESAT-6阳性细胞胞核呈蓝色荧光、胞质呈红色荧光，ESAT-6阴性细胞胞核呈蓝色荧光、胞质不着色）并分析三维成像结果。结果ESAT-6抗原主要表达于结核性脑膜炎患者脑脊液单核细胞胞质，呈红色荧光。结核性脑膜炎组有28例（80％）ESAT．6阳性、对照组仅1例（2．86％）阳性，组间差异具有统计学意义（x2=42．918，P=0．000）；ESAT-6检测灵敏度80％，特异度97．14％。结论ESAT-6抗原检测可为结核性脑膜炎的早期诊断提供一定依据，且特异性较高。LSCM技术可实现多重荧光同时观察并形成清晰的断层扫描和三维图像，把细胞学研究提高到新水平。
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